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  • A rapid and sensitive method for the detection of Xanthomonas fragariae, causal agent of angular leafspot disease in strawberry plants.

A rapid and sensitive method for the detection of Xanthomonas fragariae, causal agent of angular leafspot disease in strawberry plants.

Journal of microbiological methods (2004-07-06)
Anna Stöger, Werner Ruppitsch
ABSTRACT

Angular leaf spot is a bacterial disease caused by Xanthomonas fragariae. It has become a serious disease in the USA and Europe in recent years. Several detection procedures are described for this plant pathogen. However, they are either too time-consuming, too insensitive or impractical when handling a large number of samples routinely. Here we describe a modified protocol of the REDExtract-N-Amp Plant PCR-Kit for the detection of X. fragariae in planta and demonstrate that it provides greater sensitivity, speed and high throughput potential than methods previously described.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
REDExtract-N-Amp Plant PCR Kit, sufficient for 10 extractions, sufficient for 10 amplifications
Sigma-Aldrich
REDExtract-N-Amp Plant PCR Kit, sufficient for 1000 extractions, sufficient for 1000 amplifications
Sigma-Aldrich
REDExtract-N-Amp Plant PCR Kit, sufficient for 100 extractions, sufficient for 500 amplifications
Sigma-Aldrich
REDExtract-N-Amp Plant PCR Kit, sufficient for 100 extractions, sufficient for 100 amplifications