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  • High LET (56)Fe ion irradiation induces tissue-specific changes in DNA methylation in the mouse.

High LET (56)Fe ion irradiation induces tissue-specific changes in DNA methylation in the mouse.

Environmental and molecular mutagenesis (2014-04-12)
Florence Lima, Dacheng Ding, Wilfried Goetz, Austin J Yang, Janet E Baulch
ABSTRACT

DNA methylation is an epigenetic mechanism that drives phenotype and that can be altered by environmental exposures including radiation. The majority of human radiation exposures occur in a relatively low dose range; however, the biological response to low dose radiation is poorly understood. Based on previous observations, we hypothesized that in vivo changes in DNA methylation would be observed in mice following exposure to doses of high linear energy transfer (LET) (56) Fe ion radiation between 10 and 100 cGy. We evaluated the DNA methylation status of genes for which expression can be regulated by methylation and that play significant roles in radiation responses or carcinogenic processes including apoptosis, metastasis, cell cycle regulation, and DNA repair (DAPK1, EVL, 14.3.3, p16, MGMT, and IGFBP3). We also evaluated DNA methylation of repeat elements in the genome that are typically highly methylated. No changes in liver DNA methylation were observed. Although no change in DNA methylation was observed for the repeat elements in the lungs of these same mice, significant changes were observed for the genes of interest as a direct effect and a delayed effect of irradiation 1, 7, 30, and 120 days post exposure. At delayed times, differences in methylation profiles among genes were observed. DNA methylation profiles also significantly differed based on dose, with the lowest dose frequently affecting the largest change. The results of this study are the first to demonstrate in vivo high LET radiation-induced changes in DNA methylation that are tissue and locus specific, and dose and time dependent.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Iron, puriss. p.a., carbonyl-Iron powder, low in magnesium and manganese compounds, ≥99.5% (RT)
Iron, IRMM®, certified reference material, 0.5 mm wire
Iron, foil, 10mm disks, thickness 0.15mm, hard, 99.5%
Iron, foil, 15mm disks, thickness 0.015mm, 99.99+%
Iron, foil, not light tested, 150x150mm, thickness 0.008mm, 99.85%
Iron, foil, not light tested, 100x100mm, thickness 0.006mm, 99.85%
Iron, foil, not light tested, 50x50mm, thickness 0.001mm, 99.85%
Iron, tube, 200mm, outside diameter 12.0mm, inside diameter 10mm, wall thickness 1.0mm, annealed, 99.5%
Iron, foil, 50x50mm, thickness 0.20mm, hard, 99.5%
Iron, foil, 25x25mm, thickness 2mm, as rolled, 99.99+%
Iron, tube, 200mm, outside diameter 10.0mm, inside diameter 7mm, wall thickness 1.5mm, annealed, 99.5%
Iron, wire reel, 1m, diameter 0.5mm, as drawn, 99.998%
Iron, wire reel, 0.5m, diameter 1.0mm, as drawn, 99.99+%
Iron, wire reel, 1m, diameter 1.0mm, as drawn, 99.99+%
Iron, tube, 200mm, outside diameter 2.0mm, inside diameter 1.6mm, wall thickness 0.2mm, as drawn, 99.5%
Iron, foil, 50x50mm, thickness 0.5mm, hard, 99.5%
Iron, wire reel, 5m, diameter 0.25mm, hard, 99.99+%
Iron, foil, 300x300mm, thickness 0.20mm, hard, 99.5%
Iron, wire reel, 100m, diameter 0.065mm, hard, 99.5%
Sigma-Aldrich
Iron, nanopowder, 35-45 nm particle size, 99.5% trace metals basis
Iron, tube, 500mm, outside diameter 6.35mm, inside diameter 5.35mm, wall thickness 0.5mm, as drawn, 99.8%
Iron, foil, 10mm disks, thickness 0.003mm, 99.85%
Iron, tube, 500mm, outside diameter 2.38mm, inside diameter 1.6mm, wall thickness 0.39mm, as drawn, 99.8+%
Iron, tube, 200mm, outside diameter 3.18mm, inside diameter 1.96mm, wall thickness 0.61mm, as drawn, 99.5%
Iron, foil, 10mm disks, thickness 0.0125mm, 99.85%
Iron, wire reel, 0.5m, diameter 0.13mm, hard, 99.99+%
Iron, tube, 200mm, outside diameter 12.0mm, inside diameter 9mm, wall thickness 1.5mm, annealed, 99.5%
Iron, foil, 50x50mm, thickness 0.25mm, as rolled, 99.99+%
Iron, foil, 10m coil, thickness 0.038mm, hard, 99.5%
Iron, tube, 500mm, outside diameter 8.0mm, inside diameter 5mm, wall thickness 1.5mm, annealed, 99.5%