Role of rhBMP-2 and rhBMP-7 in the metabolism and differentiation of osteoblast-like cells cultured on chemically modified titanium surfaces.

Role of rhBMP-2 and rhBMP-7 in the metabolism and differentiation of osteoblast-like cells cultured on chemically modified titanium surfaces.

The Journal of oral implantology (2014-12-17)

Fabiano Ribeiro Cirano, Adriane Yaeko Togashi, Márcia Martins Marques, Francisco Emílio Pustiglioni, Luiz Antônio Pugliesi Alves Lima

PMID25506660

ABSTRACT

This study analyzed the role of recombinant human bone morphogenetic protein 2 (rhBMP-2) and recombinant human bone morphogenetic protein 7 (rhBMP-7) in the adhesion and differentiation of rat osteoblast-like (osteo-1) cells cultured on chemically modified titanium surfaces. Osteo-1 cells were cultured on chemically modified (modified sandblasted and acid-etched) titanium surfaces in 3 different types of medium: control, medium supplemented with 20 ng/mL rhBMP-2, and medium supplemented with 20 ng/mL rhBMP-7. The following parameters were evaluated: cell adhesion after 24 hours; total protein content; collagen content and alkaline phosphatase (AP) activity after 7, 14, and 21 days; and calcified nodule formation after 21 days. The addition of rhBMP-2 or rhBMP-7 did not influence cell adhesion (P = .1175). Cell differentiation was influenced by rhBMP-2, as demonstrated by a significant increase in collagen content after 7 days of culture (P < .0001) and a significant decrease in AP activity after 21 days (P < .0001). The addition of rhBMP-7 only influenced AP activity, and a significant increase was observed after 21 days (P < .0001). Within the limitations of the study, we conclude that the presence of rhBMP-2 or rhBMP-7 did not influence cell adhesion to chemically modified titanium surfaces but provided an additional stimulus during the differentiation of rat osteo-1 cells cultured on this type of surface.

MATERIALS

Product Number

Brand

Product Description

C3867

Sigma-Aldrich

Collagen, Type I solution from rat tail, BioReagent, suitable for cell culture, sterile-filtered

GF00761217

Titanium, wire reel, 200m, diameter 0.25mm, as drawn, 99.6+%

GF47404201

Titanium, wire reel, 5m, diameter 0.20mm, annealed, 99.6+%

GF91672139

Titanium, wire reel, 20m, diameter 0.05mm, hard, 99.8%

GF34996685

Titanium, wire reel, 200m, diameter 0.125mm, annealed, 99.6+%

GF91204466

Titanium, wire reel, 5m, diameter 1.0mm, as drawn, 99.6+%

GF43403431

Titanium, wire reel, 500m, diameter 0.20mm, annealed, 99.6+%

GF36425707

Titanium, wire reel, 200m, diameter 0.20mm, annealed, 99.6+%

GF31085760

Titanium, wire reel, 5m, diameter 0.25mm, as drawn, 99.99+%

GF46592949

Titanium, wire, straight, 1000mm, diameter 1.0mm, as drawn, 99.6+%

GF43519945

Titanium, wire reel, 2m, diameter 1.0mm, as drawn, 99.99+%

GF96391402

Titanium, wire reel, 25m, diameter 0.20mm, annealed, 99.6+%

GF55775975

Titanium, wire reel, 25m, diameter 0.25mm, as drawn, 99.6+%

GF14377631

Titanium, wire reel, 25m, diameter 0.25mm, annealed, 99.6+%

GF51690663

Titanium, wire reel, 5m, diameter 0.5mm, as drawn, 99.6+%

GF50624434

Titanium, wire reel, 200m, diameter 0.25mm, annealed, 99.6+%

GF29584187

Titanium, tube, 500mm, outside diameter 2.03mm, inside diameter 1.55mm, wall thickness 0.24mm, annealed, 99.6+%

GF90731505

Titanium, tube, 500mm, outside diameter 0.51mm, inside diameter 0.35mm, wall thickness 0.08mm, annealed, 99.6+%

GF97204990

Titanium, wire reel, 100m, diameter 0.25mm, annealed, 99.6+%

GF71783354

Titanium, wire reel, 100m, diameter 0.75mm, as drawn, 99.6+%

GF95731960

Titanium, tube, 500mm, outside diameter 3.0mm, inside diameter 1.5mm, wall thickness 0.75mm, annealed, 99.6+%

GF08595119

Titanium, wire reel, 100m, diameter 1.0mm, annealed, 99.6+%

GF71020235

Titanium, wire reel, 0.5m, diameter 1.0mm, as drawn, 99.6+%

GF16213553

Titanium, wire reel, 10m, diameter 0.125mm, annealed, 99.6+%

GF16602768

Titanium, wire reel, 1m, diameter 0.5mm, as drawn, 99.99+%

GF72193114

Titanium, tube, 500mm, outside diameter 2.03mm, inside diameter 1.63mm, wall thickness 0.2mm, annealed, 99.6+%

GF23860144

Titanium, tube, 500mm, outside diameter 6.35mm, inside diameter 4.57mm, wall thickness 0.89mm, annealed, 99.6+%

GF06813525

Titanium, wire reel, 1m, diameter 0.5mm, as drawn, 99.6+%

GF56868766

Titanium, wire reel, 25m, diameter 1.0mm, annealed, 99.6+%

GF61228403

Titanium, wire reel, 50m, diameter 0.75mm, as drawn, 99.6+%