MilliporeSigma

Phospholipids profiling and outcome of peritoneal dialysis patients.

Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals (2014-08-01)
Wen Tang, Min Li, Xin-Hong Lu, Hu-Wei Liu, Tao Wang
ABSTRACT

To investigate phospholipids (PLs) biomarkers in predicting outcome of patients undergoing peritoneal dialysis (PD). Twenty PD patients were followed using baseline plasma PLs with an improved online two-dimensional liquid chromatography-quadrupole time-of-flight mass spectrometry system. Significant differences were observed in eight PL species with sphingomyelin (SM) and glycerophosphocholine between technical survival (n=15) and failure patients (n = 5). Cox regression showed SM 21:0 (adjusted HR 13.7, 95% CI 2.42-77.88, p = 0.003) was independently associated with patients technical failure. PD failure patients had different plasma PLs profiling as compared with survival patients. Elevated plasma SM 21:0 level may potentially serve as a biomarker of PD patients at risk for adverse outcomes.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
1,2-Dimyristoyl-sn-glycero-3-phosphoethanolamine, synthetic, ≥99%
Sigma-Aldrich
Lignoceric acid, ≥99% (GC)
Sigma-Aldrich
Hexacosanoic acid, ≥95% (capillary GC)
Sigma-Aldrich
Hexacosanoic acid, technical, ≥90% (GC)
Sigma-Aldrich
Ammonium formate, reagent grade, 97%
Supelco
Ammonium formate, eluent additive for LC-MS, LiChropur, ≥99.0%
Sigma-Aldrich
Ammonium formate, BioUltra, ≥99.0% (calc. based on dry substance, NT)
Sigma-Aldrich
Ammonium formate, ≥99.995% trace metals basis
Supelco
Ammonium formate solution, 10 mM in H2O, suitable for HPLC
Sigma-Aldrich
Ammonium formate solution, BioUltra, 10 M in H2O