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  • Immortalised human mesenchymal stem cells undergo chondrogenic differentiation in alginate and PGA/PLLA scaffolds.

Immortalised human mesenchymal stem cells undergo chondrogenic differentiation in alginate and PGA/PLLA scaffolds.

Cell and tissue banking (2014-05-17)
K Stölzel, G Schulze-Tanzil, H Olze, S Schwarz, E M Feldmann, N Rotter
ABSTRACT

Adult mesenchymal stem cells (MSCs) are a promising cell source in tissue engineering due to their availability, ease of isolation and high proliferative activity. This study was undertaken to investigate whether immortalised human MSC are able to undergo chondrogenic differentiation when cultured in alginate or in resorbable scaffolds. We directly compared chondrogenesis MSCs with that of human nasoseptal chondrocytes. Two previously established human stem cell lines L87/4 and V54-2 immortalised using the SV40 large T-antigen were either cultured in alginate or in polyglycolic acid/poly-L-lactic acid (PGA/PLLA) (90/10) copolymer scaffolds. TGF-β1 was added for induction of chondrogenesis. Human nasoseptal chondrocytes and human fibroblasts were used as controls. Cultures were analysed for sulfated glycosaminoglycans (alcian blue staining) and for the presence of collagen type I, II and X (immunolabelling). SV40 large T-antigen immortalised human MSCs have the potential to undergo chondrogenic differentiation: After 21 days, cartilage-specific type II collagen was present in alginate and PGA/PLLA scaffolds, independent of the addition of TGF-β1. Collagen type X was present in monolayer cultures as well as in alginate and PGA/PLLA scaffolds. Collagen type I was produced in marginal amounts only. Immortalised human MSCs are a suitable tool to study chondrogenesis in vitro and to screen biomaterials for cartilage tissue engineering applications.

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Sigma-Aldrich
Aluminum sulfate, 99.99% trace metals basis
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25 g
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₩116,421
100 g
In Stock
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₩233,450
250 g
In Stock
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₩516,432
500 g
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₩963,641
1 kg
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₩1,612,125
5 kg
In Stock
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₩5,589,402
25 kg
In Stock
Details...
₩11,934,066
50 kg
Estimated to ship on February 03, 2025
Details...
₩22,508,031
Sigma-Aldrich
Aluminum sulfate, meets USP testing specifications
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SKUPack SizeAvailabilityPriceQuantity
25 g
In Stock
Details...
₩116,421
100 g
In Stock
Details...
₩233,450
250 g
In Stock
Details...
₩516,432
500 g
In Stock
Details...
₩963,641
1 kg
In Stock
Details...
₩1,612,125
5 kg
In Stock
Details...
₩5,589,402
25 kg
In Stock
Details...
₩11,934,066
50 kg
Estimated to ship on February 03, 2025
Details...
₩22,508,031
Sigma-Aldrich
Fluorescein diacetate, used as cell viability stain
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SKUPack SizeAvailabilityPriceQuantity
25 g
In Stock
Details...
₩116,421
100 g
In Stock
Details...
₩233,450
250 g
In Stock
Details...
₩516,432
500 g
In Stock
Details...
₩963,641
1 kg
In Stock
Details...
₩1,612,125
5 kg
In Stock
Details...
₩5,589,402
25 kg
In Stock
Details...
₩11,934,066
50 kg
Estimated to ship on February 03, 2025
Details...
₩22,508,031