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  • Cytochrome P450 3A4 and 2D6-mediated metabolism of leisure and medicinal teas.

Cytochrome P450 3A4 and 2D6-mediated metabolism of leisure and medicinal teas.

Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques (2014-09-17)
Teresa W Tam, Rui Liu, Ammar Saleem, John Thor Arnason, Anthony Krantis, Brian C Foster
ABSTRACT

Thirty-five commercially available Camellia sinensis (black and green) and herbal leisure teas and an assortment of Traditional Chinese medicinal teas were randomly selected and examined for their potential to inhibit the drug metabolizing enzyme cytochrome P450 3A4 (CYP3A4). The study was then extended to examine CYP2D6*1 and CYP2D6*10. Microtiter fluorometric assays were utilized to examine the potential for the teas to inhibit CYP-mediated metabolism. Aqueous or alcoholic extracts of the dried tea plant material were examined. Most of the black and green leisure teas generally inhibited CYP3A4 more than the Chinese medicinal teas. The medicinal Chinese teas were generally more inhibitory towards CYP3A4 compared to the CYP2D6 isozymes, and the aqueous extracts displayed more potency than the alcoholic extracts. Tea whether used for leisure or medicinal purposes has the potential to inhibit CYP3A4-mediated drug metabolism particularly black tea.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Quinidine, anhydrous
USP
Ketoconazole, United States Pharmacopeia (USP) Reference Standard
Supelco
Ketoconazole, Pharmaceutical Secondary Standard; Certified Reference Material
Ketoconazole, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Ketoconazole, 99.0-101.0% (EP, titration)
Sigma-Aldrich
Quinidine, crystallized, ≥98.0% (dried material, NT)
Sigma-Aldrich
Ketoconazole