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  • miR-219 inhibits the proliferation, migration and invasion of medulloblastoma cells by targeting CD164.

miR-219 inhibits the proliferation, migration and invasion of medulloblastoma cells by targeting CD164.

International journal of molecular medicine (2014-04-24)
Jia-Ang Shi, Da-Lin Lu, Xuan Huang, Wei Tan
ABSTRACT

It is known that microRNA-219 (miR-219) expression is downregulated in medulloblastoma. In the present study, we investigated the expression, targets and functional effects of miR-219 in D283-MED medulloblastoma cells. We first demonstrated that miR-219 not only inhibits proliferation, but also suppresses the invasion and migration of D283-MED cells. Moreover, the knockdown of miR-219 promoted the proliferation, migration and invasion of the D283-MED cells. Secondly, we predicted that miR-219 targets the 3' untranslated region (3'UTR) of CD164 and orthodenticle homeobox 2 (OTX2) and then confirmed that it significantly downregulated the protein expression of CD164 and OTX2 in D283-MED cells. Finally, we demonstrated that the proliferation, invasion and migration of D283-MED cells were promoted by theectopic expression of CD164. These results indicate that miR-219 suppresses the proliferation, migration and invasion of medulloblastoma cells by targeting CD164. The results also suggest that miR-219 may serve as a potential therapeutic agent for medulloblastoma.

MATERIALS
Product Number
Brand
Product Description

Roche
Cell Proliferation ELISA, BrdU (colorimetric), sufficient for ≤1,000 tests