Skip to Content
MilliporeSigma
  • Effect of shortening or omitting the dry period of Holstein-Friesian cows on casein composition of milk.

Effect of shortening or omitting the dry period of Holstein-Friesian cows on casein composition of milk.

Journal of dairy science (2015-10-12)
Ruben de Vries, Ariëtte van Knegsel, Monika Johansson, Helena Lindmark-Månsson, Toon van Hooijdonk, Kjell Holtenius, Kasper Hettinga
ABSTRACT

The aim of this study was to evaluate the effect of shortening or omitting the dry period of dairy cows on milk casein composition. For this study, we analyzed milk samples of 90 cows with a dry period of 0, 30, or 60d and either a glucogenic or a lipogenic ration in early lactation. Milk was sampled at 6 and 2wk prepartum and at 2, 6, and 12wk postpartum. Milk was analyzed for casein (CN) composition by capillary zone electrophoresis, and isoforms of κ-CN were measured by reversed phase-HPLC. Shortening the dry period from 60 to 30d reduced the αS1-CN fraction by 3.8% and increased the αS2-CN fraction by 5.5%. In milk from cows with a 0-d dry period, the glycosylated κ-CN fraction in late lactation increased from 8 to 12% between 6 and 2wk prepartum. After calving, the glycosylated κ-CN fraction in milk was higher for cows with a 0-d dry period (6.7%) compared with cows with a 60-d dry period (5.2%). The glycosylated κ-CN fraction at 2wk postpartum was negatively correlated with milk yield, suggesting that glycosylation was related to reduced productivity of mammary epithelial cells. In early lactation, the β-CN fraction was reduced in milk of cows with a 0-d dry period. A lowered β-CN fraction was associated with high somatic cell count and greater parity, indicating that it was the result of proteolytic activity. In conclusion, casein composition changes that result from shortening the dry period from 60 to 30d are not expected to affect processing characteristics of milk. Applying a 0-d dry period may affect processability of milk because of a higher glycosylated κ-CN fraction, and possibly because of higher proteolytic activity compared with a 60-d dry period.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Trisodium citrate dihydrate, meets USP testing specifications
Sigma-Aldrich
Sodium citrate tribasic dihydrate, ≥98%
Sigma-Aldrich
Sodium citrate tribasic dihydrate, for molecular biology, ≥99%
Sigma-Aldrich
Sodium citrate tribasic dihydrate, BioUltra, for molecular biology
Sigma-Aldrich
Sodium citrate tribasic dihydrate, JIS special grade, ≥99.0%
Sigma-Aldrich
Trifluoroacetic acid, SAJ special grade, ≥99.0%
Sigma-Aldrich
Sodium citrate tribasic dihydrate, ACS reagent, ≥99.0%
Sigma-Aldrich
Sodium citrate dihydrate, ≥99%, FG
Sigma-Aldrich
Trifluoroacetic acid, ReagentPlus®, 99%
Sigma-Aldrich
Trifluoroacetic acid, ≥99%, for protein sequencing
Sigma-Aldrich
Trifluoroacetic acid, puriss. p.a., suitable for HPLC, ≥99.0% (GC)