Skip to Content
MilliporeSigma

Pin1At regulates PIN1 polar localization and root gravitropism.

Nature communications (2016-01-23)
Wanyan Xi, Ximing Gong, Qiaoyun Yang, Hao Yu, Yih-Cherng Liou
ABSTRACT

Root gravitropism allows plants to establish root systems and its regulation depends on polar auxin transport mediated by PIN-FORMED (PIN) auxin transporters. PINOID (PID) and PROTEIN PHOSPHATASE 2A (PP2A) act antagonistically on reversible phosphorylation of PINs. This regulates polar PIN distribution and auxin transport. Here we show that a peptidyl-prolyl cis/trans isomerase Pin1At regulates root gravitropism. Downregulation of Pin1At suppresses root agravitropic phenotypes of pp2aa and 35S:PID, while overexpression of Pin1At affects root gravitropic responses and enhances the pp2aa agravitropic phenotype. Pin1At also affects auxin transport and polar localization of PIN1 in stele cells, which is mediated by PID and PP2A. Furthermore, Pin1At catalyses the conformational change of the phosphorylated Ser/Thr-Pro motifs of PIN1. Thus, Pin1At mediates the conformational dynamics of PIN1 and affects PID- and PP2A-mediated regulation of PIN1 polar localization, which correlates with the regulation of root gravitropism.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Murashige and Skoog Basal Salt Mixture (MS), powder, suitable for plant cell culture
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 L
1 Available to ship on January 09, 2025
Details...
$15.50
10 L
1 Available to ship on January 09, 2025
Details...
$49.90
50 L
1 Estimated to ship on March 09, 2025
$176.00
Sigma-Aldrich
Agar, microbiology tested, suitable for plant cell culture, suitable for cell culture, powder
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 g
1 Available to ship on January 09, 2025
Details...
$78.60
500 g
1 Available to ship on January 09, 2025
Details...
$257.00
1 kg
1 Available to ship on January 09, 2025
Details...
$396.00
5 kg
1 Available to ship on January 09, 2025
Details...
$1,520.00
10 kg
1 Available to ship on January 09, 2025
Details...
$2,220.00
25 kg
1 Available to ship on January 09, 2025
Details...
$4,020.00