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  • Hepatic differentiation of human pluripotent stem cells on human liver progenitor HepaRG-derived acellular matrix.

Hepatic differentiation of human pluripotent stem cells on human liver progenitor HepaRG-derived acellular matrix.

Experimental cell research (2016-02-09)
Liisa K Kanninen, Pauliina Porola, Johanna Niklander, Melina M Malinen, Anne Corlu, Christiane Guguen-Guillouzo, Arto Urtti, Marjo L Yliperttula, Yan-Ru Lou
ABSTRACT

Human hepatocytes are extensively needed in drug discovery and development. Stem cell-derived hepatocytes are expected to be an improved and continuous model of human liver to study drug candidates. Generation of endoderm-derived hepatocytes from human pluripotent stem cells (hPSCs), including human embryonic stem cells and induced pluripotent stem cells, is a complex, challenging process requiring specific signals from soluble factors and insoluble matrices at each developmental stage. In this study, we used human liver progenitor HepaRG-derived acellular matrix (ACM) as a hepatic progenitor-specific matrix to induce hepatic commitment of hPSC-derived definitive endoderm (DE) cells. The DE cells showed much better attachment to the HepaRG ACM than other matrices tested and then differentiated towards hepatic cells, which expressed hepatocyte-specific makers. We demonstrate that Matrigel overlay induced hepatocyte phenotype and inhibited biliary epithelial differentiation in two hPSC lines studied. In conclusion, our study demonstrates that the HepaRG ACM, a hepatic progenitor-specific matrix, plays an important role in the hepatic differentiation of hPSCs.

MATERIALS
Product Number
Brand
Product Description

Millipore
Accutase cell detachment solution, A cell detachment solution of proteolytic & collagenolytic enzymes. The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.