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  • Complex contributions of fibronectin to initiation and maturation of microfibrils.

Complex contributions of fibronectin to initiation and maturation of microfibrils.

The Biochemical journal (2013-09-28)
Laetitia Sabatier, Jelena Djokic, Christine Fagotto-Kaufmann, Marian Chen, Douglas S Annis, Deane F Mosher, Dieter P Reinhardt
ABSTRACT

Fibrillins constitute the backbone of extracellular multifunctional assemblies present in elastic and non-elastic matrices, termed microfibrils. Assembly of fibrillins into microfibrils and their homoeostasis is poorly understood and is often compromised in connective tissue disorders such as Marfan syndrome and other fibrillinopathies. Using interaction mapping studies, we demonstrate that fibrillins require the complete gelatin-binding region of fibronectin for interaction, which comprises domains FNI6-FNI9. However, the interaction of fibrillin-1 with the gelatin-binding domain of fibronectin is not involved in fibrillin-1 network assembly mediated by human skin fibroblasts. We show further that the fibronectin network is essential for microfibril homoeostasis in early stages. Fibronectin is present in extracted mature microfibrils from tissue and cells as well as in some in situ microfibrils observed at the ultrastructural level, indicating an extended mechanism for the involvement of fibronectin in microfibril assembly and maturation.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Monoclonal Anti-Fibronectin antibody produced in mouse, clone FN-15, ascites fluid
Sigma-Aldrich
Anti-Fibronectin Antibody, Chemicon®, from rabbit