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  • A high-yielding serum-free, suspension cell culture process to manufacture recombinant adenoviral vectors for gene therapy.

A high-yielding serum-free, suspension cell culture process to manufacture recombinant adenoviral vectors for gene therapy.

Cytotechnology (2008-11-13)
G Schoofs, T J Monica, J Ayala, J Horwitz, T Montgomery, G Roth, F J Castillo
ABSTRACT

We have developed an efficient, reproducible, and scaleable cell culture process for a recombinant adenoviral vector expressing therapeutic transgenes for clinical trials. HEK 293 cells - which support the propagation of E1 deficient adenovirus - were first adapted to serum free media and suspension growth. Subsequent studies focused on the infection, virus production and harvest from suspension culture bioreactors. Future studies are planned to address the kinetics of adenovirus production in HEK 293 as well as in other cell lines.

MATERIALS
Product Number
Brand
Product Description

SAFC
EX-CELL® 293 Serum-Free Medium for HEK 293 Cells, Animal-protein free, with L-glutamine, without sodium bicarbonate, dry powder, suitable for cell culture
SAFC
EX-CELL® 293 Serum-Free Medium for HEK 293 Cells, Serum-free, Animal-protein free, without L-glutamine, liquid, suitable for cell culture