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  • Production of protein kinase C-delta by the baculovirus-insect cell system in serum-supplemented and serum-free media.

Production of protein kinase C-delta by the baculovirus-insect cell system in serum-supplemented and serum-free media.

Journal of bioscience and bioengineering (2005-10-20)
Hideki Yamaji, Daigo Hirakawa, Shin-Ichi Tagai, Hideki Fukuda
ABSTRACT

When rat protein kinase C-delta (PKC-delta) was produced by Sf9 cells infected with a recombinant baculovirus in shake-flask culture using a serum-containing medium, the intracellular PKC-delta content decreased in the late period while the extracellular PKC-6 markedly increased. During the late period of serum-free culture, the extracellular PKC-6 level considerably declined, but the addition of a protease inhibitor, leupeptin, prevented the reduction in PKC-delta production.

MATERIALS
Product Number
Brand
Product Description

SAFC
EX-CELL® 420 Serum-Free Medium for Insect Cells, Protein-free, without L-glutamine, liquid, sterile-filtered, suitable for insect cell culture
SAFC
EX-CELL® 420 Serum-Free Medium for Insect Cells, with L-glutamine, without sodium bicarbonate, dry powder, suitable for insect cell culture
SAFC
EX-CELL® 420 Serum-Free Medium for Insect Cells, with L-glutamine, sterile-filtered, suitable for insect cell culture