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  • Rapid method for the radioisotopic analysis of gaseous end products of anaerobic metabolism.

Rapid method for the radioisotopic analysis of gaseous end products of anaerobic metabolism.

Applied microbiology (1974-08-01)
D R Nelson, J G Zeikus
ABSTRACT

A gas chromatographic procedure for the simultaneous analysis of (14)C-labeled and unlabeled metabolic gases from microbial methanogenic systems is described. H(2), CH(4), and CO(2) were separated within 2.5 min on a Carbosieve B column and were detected by thermal conductivity. Detector effluents were channeled into a gas proportional counter for measurement of radioactivity. This method was more rapid, sensitive, and convenient than gas chromatography-liquid scintillation techniques. The gas chromatography-gas proportional counting procedure was used to characterize the microbial decomposition of organic matter in anaerobic lake sediments and to monitor (14)CH(4) formation from H(2) and (14)CO(2) by Methanosarcina barkeri.

MATERIALS
Product Number
Brand
Product Description

Supelco
Carbosieve Adsorbent, matrix Carbosieve G, 80-100 mesh, bottle of 5 g
Supelco
Carbosieve Adsorbent, matrix Carbosieve S-III, 60-80 mesh, bottle of 10 g
Supelco
Carbosieve Adsorbent, matrix Carbosieve S-II, 60-80 mesh, bottle of 10 g
Supelco
Carbosieve Adsorbent, matrix Carbosieve S-II, 80-100 mesh, bottle of 10 g
Supelco
Carbosieve Adsorbent, matrix Carbosieve G, 60-80 mesh, bottle of 5 g
Supelco
Carbosieve SIII, stainless steel TD tube, O.D. × I.D. × L 6 mm × 4 mm × 7 in., preconditioned, pkg of 1 ea