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  • Evidence for the Initial Steps of DHA Biohydrogenation by Mixed Ruminal Microorganisms from Sheep Involves Formation of Conjugated Fatty Acids.

Evidence for the Initial Steps of DHA Biohydrogenation by Mixed Ruminal Microorganisms from Sheep Involves Formation of Conjugated Fatty Acids.

Journal of agricultural and food chemistry (2018-01-02)
Noelia Aldai, Pierluigi Delmonte, Susana P Alves, Rui J B Bessa, John K G Kramer
ABSTRACT

Incubation of DHA with sheep rumen fluid resulted in 80% disappearance in 6 h. The products were analyzed as their fatty acid (FA) methyl esters by GC-FID on SP-2560 and SLB-IL111 columns. The GC-online reduction × GC and GC-MS techniques demonstrated that all DHA metabolites retained the C22 structure (no evidence of chain-shortening). Two new transient DHA products were identified: mono-trans methylene interrupted-DHA and monoconjugated DHA (MC-DHA) isomers. Identification of MC-DHA was confirmed by their predicted elution using equivalent chain length differences from C18 FA, their molecular ions, and the 22:5 products formed which were the most abundant at 6 h. The 22:5 structures were established by fragmentation of their 4,4-dimethyloxazoline derivatives, and all 22:5 products contained an isolated double bond, suggesting formation via MC-DHA. The most abundant c4,c7,c10,t14,c19-22:5 appeared to be formed by unknown isomerases. Results suggest that the initial biohydrogenation of DHA was analogous to that of C18 FA.

MATERIALS
Product Number
Brand
Product Description

Supelco
SLB®-IL111 Capillary GC Column, L × I.D. 100 m × 0.25 mm, df 0.20 μm
Supelco
SLB®-IL111 Capillary GC Column, L × I.D. 30 m × 0.25 mm, df 0.20 μm
Supelco
SLB®-IL111 Capillary GC Column, L × I.D. 200 m × 0.25 mm, df 0.20 μm
Supelco
SLB®-IL111 Capillary GC Column, L × I.D. 60 m × 0.25 mm, df 0.20 μm
Supelco
SP®-2560 Capillary GC Column, L × I.D. 200 m × 0.25 mm, df 0.20 μm
Supelco
SLB®-5ms Capillary GC Column, L × I.D. 30 m × 0.25 mm, df 0.10 μm
Supelco
SLB®-IL111i Capillary GC Column, L × I.D. 30 m × 0.25 mm, df 0.20 μm
Supelco
SLB®-IL111i Capillary GC Column, L × I.D. 60 m × 0.25 mm, df 0.20 μm
Supelco
SLB®-5ms Capillary GC Column, L × I.D. 60 m × 0.25 mm, df 0.25 μm
Supelco
SLB®-5ms Capillary GC Column, L × I.D. 15 m × 0.10 mm, df 0.10 μm
Supelco
SLB®-5ms Capillary GC Column, L × I.D. 10 m × 0.10 mm, df 0.10 μm
Supelco
SP®-2560 Capillary GC Column, L × I.D. 75 m × 0.18 mm, df 0.14 μm
Supelco
SLB®-5ms Capillary GC Column, L × I.D. 30 m × 0.25 mm, df 1.00 μm
Supelco
SLB®-5ms Capillary GC Column, L × I.D. 15 m × 0.25 mm, df 0.25 μm
Supelco
SLB®-5ms Capillary GC Column, L × I.D. 30 m × 0.32 mm, df 0.25 μm
Supelco
SP®-2560 Capillary GC Column, L × I.D. 100 m × 0.25 mm, df 0.20 μm
Supelco
SLB®-5ms Capillary GC Column, L × I.D. 20 m × 0.18 mm, df 0.18 μm
Supelco
SLB®-5ms Capillary GC Column, L × I.D. 30 m × 0.25 mm, df 0.50 μm
Supelco
SP®-2560 Capillary GC Column, L × I.D. 100 m × 0.25 mm, df 0.20 μm
Supelco
SLB®-5ms Capillary GC Column, L × I.D. 30 m × 0.25 mm, df 0.25 μm