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Salmonella Detection Procedure (EN ISO 6579-1)

The International Organization for Standardization (ISO) published the new EN ISO 6579-1 standard in 2017, which specifies a horizontal method for the detection of Salmonella spp. in the food production chain.

Like the preceding ISO 6579:2002+Amd 1:2007, the latest ISO 6579-1:2017 covers the testing of products intended for human consumption, animal feeding and environmental samples in food production and handling.

In addition, EN ISO 6579-1:2017 now includes testing of milk and milk products as well as samples from the primary production stage, such as animal feces, dust and boot socks.


Procedure for Salmonella detection according to EN ISO 6579-1

DayProcedure Step 
Day 1Pre-enrichmentSample + Buffered Peptone Water (BPW) Tenfold dilution
18 h (± 2) • 34 °C - 38 °C
  
Day 2Selective enrichment
0.1 mL BPW to 10 mL RVS
broth or MSRV agar
24 h (± 3) • 41.5 °C (± 1)
  1 mL BPW to
10 mL MKTTn
24 h (± 3) • 37 °C (± 1)
  
Day 3Plating-outXLD agar 24 h (± 3) • 37 °C (± 1)   +   Second isolation agar
 Growth Interpretation
Day 4, 5, 6Confirmation

Expression of result
Test at least 1 typical or suspect colony.
If negative, test additional 4 marked colonies.
Non-selective agar (e.g. Nutrient Agar)
24 h (± 3) • 34 °C - 38 °C
Biochemical/serological testing Expression of results

EN ISO 6579-1 at a Glance

  • Part one (detection) of three-part EN ISO 6579 replaces EN ISO 6579:2004+Amd 1:2007
  • EN ISO 6785:2007 (dairy products) method details now incorporated
  • Samples from primary production stage added to scope
  • Incubation temperature range for non-selective media extended to 34 – 38 °C
  • Choice of RVS or MSRV agar for selective enrichment of food, animal feed and environmental samples
  • Directions for choice of second isolation medium in addition to XLD agar
  • Only one suspect colony needed for confirmation (if negative, 4 more to be tested)
  • Optional: biochemical confirmation on well-isolated colony direct from selective plate
  • β-galactosidase and indole tests now optional, Voges-Proskauer reaction omitted
  • Detection of Salmonella Typhi and S. Paratyphi described 
  • Performance testing for the culture media in-depth  

As a worldwide leading provider of a broad range of dehydrated granulated and ready-to-use culture media for food, beverages and water microbiology, we closely track and supports the development of relevant standards aimed at increasing consumer confidence and safety.

Our GranuCult® granulated media, Readybag® pre-weighed granulated media in ready-to-use bags, ReadyPlate™ & ReadyTube® ready-to-use media are all compliant with EN ISO 11133:2014. All information regarding compliance with additional reference standards are displayed on the product label and in the product’s technical information available on our website.  

Compliance to EN ISO 6579-1

We have implemented all the requirements described in EN ISO 6579-1. The following culture media and accessories described in ISO 6579-1 are available:  

ProductPack SizeCatalog No.Other Pack Sizes Available
GranuCult® Buffered Peptone Water acc. ISO 6579, ISO 21528, ISO 22964, FDA-BAM and EP500 g1.07228.05005 kg, 25 kg
Readybag® Buffered Peptone Water acc. ISO 6579, ISO 21528, ISO 22964, FDA-BAM and EP, 5.7 g, irradiated60 bags1.0090860 bags x 29 g,
   35 bags x 86 g
ReadyTube® 225 BPW ISO 6579, ISO 6887, ISO 21528, ISO 11290, FDA-BAM and EP6 x 225 mL1.46404.00066 x 1000 mL,
   20 x 9 mL
GranuCult® RVS (RAPPAPORT-VASSILIADIS-Soya) Broth (Base) acc. ISO 6579500 g1.07700.0500 
Novobiocin Sodium Salt1 gN6160-1-G5 g, 25 g
ReadyTube® 10 RVS Broth ISO 657920 x 10 mL1.46694.0020 
GranuCult® MKTTn (MULLER KAUFFMANN Tetrathionate Novobiocin) Broth (Base) acc. ISO 6579500 g1.05878.0500 
Potassium Iodide250 g1.05043.0250 
Iodine resublimed100 g1.04761.0100 
MSRV (Modified Semi-solid RAPPAPORT-VASSILIADIS) Medium (Base) acc. ISO 6579500 g1.09878.0500 
MSRV Selective Supplement10 x 1 vial1.09874.0010 
ReadyTube® 12 MSRV Medium ISO 6579100 x 12 mL1.46622.0100 
GranuCult® XLD (Xylose Lysine Deoxycholate) agar acc. ISO 6579500 g1.05287.0500 
ReadyPlate™ XLD Agar ISO 657920 x 90 mm1.46751.0020 
RAMBACH® Agar4 x 250 mL1.07500.00014 x 1000 mL, 1 x 50 L
RAMBACH® Agar ready-to-use20 x 90 mm1.46719.0020100 x 90 mm
GranuCult® Nutrient agar acc. ISO 6579, ISO 10273 and ISO 21528500 g1.05450.0500 
Bismuth Sulfite Agar acc. WILSON-BLAIR500 g1.05418.0500 
Triple Sugar Iron Agar500 g1.03915.0500 
Urea Agar (Base) acc. CHRISTIANSEN500 g1.08492.0500 
Urea GR for Analysis ACS, Reagent Ph Eur500 g1.08487.0500 
MR-VP (Methyl Red-VOGES-PROSKAUER) Broth500 g1.05712.0500 
KOVAC’S Indole Reagent100 mL1.09293.0100 

Materials

Reagents
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Culture media and Broth
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