Expression and purification of GST-tagged proteins can be monitored by Western blot analysis, using Amersham ECL, Amersham ECL Prime, or Amersham ECL Select detection systems to enhance sensitivity.
Secondary antibody to detect the anti-GST antibody (such as anti-goat IgG HRP conjugate)
Appropriate membrane, such as Amersham Hybond™ ECL (nitrocellulose) or Amersham Hybond-P (PVDF)
Although anti-GST antibody from Cytiva has been cross-adsorbed with E. coli proteins, low levels of cross-reacting antibodies may remain. Samples of E. coli lysates that do not contain a recombinant pGEX plasmid and samples that contain the parental pGEX plasmid should always be run as controls.
Electrophoresis and protein transfer can be accomplished using a variety of equipment and reagents.
Longer incubation times with blocking/incubation buffer may reduce background signal.
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