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HPLC Discovery HS C18

Discovery HS C18 HPLC Columns, 120 Å;
3 µm, 5 µm and 10 µm particle sizes available

  • Specifically developed for pharmaceutical analysis and purification
  • Ideal for LC/MS applications - No detectable bleed (as independently tested)
  • Highly stable to ensure excellent run-to-run and lot-to-lot reproducibility and long column life
  • Scalable from analytical to prep

NO DETECTABLE LC/MS BLEED

Discovery HS C18

No TIC Baseline rise.
Essentially no m/z peaks generated from column ("x" indicates peaks also found in blank run)

Column: Discovery HS C18 and Conventional C18, 5cm x 4.6mm, 3µm;
0.1% TFA; Flow Rate: 1mL/min; Temp: ambient, esi+ ion.

Competitor C18

TIC Baseline rise can interfere with sensitivity, and quantitation measurements. Bleed m/z peaks could coincidently interfere with "real" peaks.

Mobile Phase: 15 min. gradient from 0-100% MeOH in

(work conducted by Dr. Shane Needham, Alturas Analytics, Moscow, ID)

(work conducted by Dr. Shane Needham, Alturas Analytics, Moscow, ID)

LOT TO LOT REPRODUCIBILITY OF DISCOVERY HS C18

Column: Discovery HS C18, 15 cm x 4.6 mm, 5 µm
Mobile Phase: 80% 10 mM ammonium acetate, pH 6.8 : 20% MeOH
Flow Rate: 1 mL/min
Temp: 35 °C
Detection: 254 nm;
Injection: 1 µL

1. Uracil (8 µg/mL)
2. Procainamide (30 µg/mL)
3. Sorbic acid (4 µg/mL)
4. Pyridine (100 µg/mL)
5. Caffeine (40 µg/mL)
6. Phenol (240 µg/mL)

g001597_9

GREATER HYDROPHOBIC RETENTION COMPARED TO OTHER POPULAR C18 PHASES

Columns: 7.5 cm x 4.6 mm, 3 µm/3.5 µm
Mobile Phase: 35:65 - H2O:ACN
Flow Rate: 2 mL/min; Detection: 254 nm

g001500


Ordering Information:

3µm Discovery HS C18 HPLC Columns
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5µm Discovery HS C18 HPLC Columns
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10µm Discovery HS C18 HPLC Columns
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2cm Supelguard Cartridges with Discovery HS C18 Packings
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* 569275-U (Kit includes cartridge, stand-alone holder, tubing, 2 nuts and ferrules)

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