USP HPLC Analysis of L-Methionine on Ascentis® Express OH5
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Conditions | |
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column | Ascentis Express OH5 15 cm x 4.6 mm, 5 µm particles (50347-U) |
column temp. | 40 °C |
mobile phase | [A] 0.005M Ammounium formate in Water (pH: 3.15 with Trifluoroacetic Acid) ), [B] Acetonitrile (30:70, A:B) |
flow rate | 1.0 mL/min |
pressure | 914 psi (63 bar) |
sample | 0.8 mg/mL water:acetonitrile (1:1) |
injection | 3 µL |
detector | Agilent 380-ELSD |
Description | |
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Analysis Note | This USP application was generated by RTC on an Ascentis® Express OH5 column. |
Categories | Analytical Chromatography, Amino Acids |
Featured Industry | Life Science and Biopharma |
suitability | application for HPLC |
Materials
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