Figure 1.Growth curve graph of a Caco-2 cell culture (A). Zooming into the graph allows one to see the axis more clearly (B and C) and calculate the time it takes for the cell confluency to double (for example, from 30% to 60%).
Figure 2.Steps to narrow the selection of images in a profile.
Figure 3.Downloading data from a project.
Figure 4.Delete Cell Line screen.
Figure 5.Delete Project screen.
When masking doesn’t recognize all the cells for certain cell types, it can lead to a lower confluency reading. To correct this:
Yes, this helps to look at growth curves under different passages.
If you adjust the focus and make the cells appear as dots (i.e., dark circles), the Millicell® DCI will count more of the cells in the quadrant (see Figure 6C vs. 6A). In Figure 6A, the cells are seen as bright circles. The Millicell® DCI counted 16 cells in the grid (Figure 6B). By adjusting the focus and making the cells appear as dark circles (Figure 6C), the Millicell® DCI counts 44 cells within the same grid (Figure 6D).
Figure 6.Optimizing hemocytometer count by adjusting focus.
You can use the “Notes” section to record specific information such as % confluency, passage number, etc. This allows you to save images in the specific project folder you need that is easily accessed.
If you want to get a snapshot of estimated cell count results across multiple days, under the “Trends” section you can change the statistic to “Estimated count” instead of “Confluency”. Please note that this will give you an estimated cell count.
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