Using lentivirus as a means to deliver shRNAs has become standard practice in many labs exploring RNAi. Infection (transduction) with VSV-G pseudotyped lentivirus allows for simple, efficient delivery into a wide variety of cell types, including: non-dividing, primary, and stem cells. In the few instances when a particular cell type is difficult to transduce, there are multiple methods to enhance delivery, such as: Spinoculation or magnetic nanoparticle beads (ExpressMag™ Transduction System).
The table below is designed to aid in your lentivirus transduction experiments when working with a given cell line. All validated cell lines are a result of researchers using the RNAi Consortium (TRC) shRNA library delivered via lentivirus. If you fail to find your cell line of interest, it is suggested to identify similar cell lines that may act as a good starting point for your own research. Transduction conditions are presented in one of two ways on this table.
There may be multiple literature references to a single cell type. It is encouraged to review multiple references to best gauge the best transduction conditions for your model.
Some cells will have specific transduction conditions already populated within the table. These conditions were experimentally determined from either the Broad Institute, our internal R&D, or external collaborators. While these conditions worked well for a given researcher, they are meant to act as a starting point in your own work.
If the source is the our MISSION® Team or the TRC-Broad Institute, then the virus titer was between 106 and 107.
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