We are committed to help unlock the today’s challenge of releasing the energy of glucose that nature has cleverly locked into lignocellulosic biomass. To the untrained eye, starch and cellulose structures appear almost identical. Yet the barriers between cellulosic biomass hydrolysis and ethanol production have proven to be orders of magnitude more challenging than with starch-like biomass.
Although many of the biochemical aspects of these challenges are well understood, they remain an enigma of nature. The two main, and closely related hinderences to the enzymatic hydrolysis of cellulosic polymers are; How do we physically dismantle biomass on a molecular level to yield cellulosic polymers available to hydrolysis by cellulase and other glycolytic enzymes? How do we penetrate the barrier posed by the closely integrated network of liginins surrounding cellulose microfibrils?
Many commercial cellulase preparations contain various classes of cellulases and related activities.
Cerial β-Glucan, Polymer of β-(1,4) glycopyranosyl units occring as predomiantly cellotriose and cellotetraose separated by single β-(1,3)-Dglycopyranosyl units. Cross-linking can occur within the consecutive cellotriose regions.
from Aspergillus sp.
Product Number C2605 (Carezyme® 1000 L)
from Aspergillus niger
Product Number C1184 (powder, min. 0.3 un/mg)
from Trichoderma reesei
Product Number C8546 (powder, min. 1.0 un/mg)
Product Number C2730 (Celluclast® 1.5 L)
from Trichoderma viride
Product Number C0615 (Yakult Onozuka RS)
Product Number C1794 (cell culture tested, powder, min. 3.0-10 un/mg)
from Hypocrea jecorina
Product Number E6412 (recombinant expressed in corn)
from Basidiomycetes sp.
Product Number D9515 (Crude powder containing laminarinase, xylanase and cellulase)
from Acidothermus cellulolyticus
Product Number E2164 (recombinant, expressed in corn)
Laminarinase catalyzes the endohydrolysis of 1,3- or 1,4-linkages in β-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolysed is itself substituted at C-3
Xylan Hemicellulose, Polymer of β-(1,4)-D-xylopyranosyl units
Hemicelluloses are a group of plant-derived heteropolysaccharides associated with cellulose and lignin. The most common hemicelluloses are: xylan, glucuronoxylan, arabinoxylan, glucomannan and xyloglucan. In angiosperms, the principal hemicellulose component, xylan, is a polymer of β(1-4)D-xylopyranose. In arabinoxylan, branching occurs at the C2 & C3 postions with α-L-arabinofunaose. Glucuronoxylan, also found in angiosperms, has the xylan backbone with 4-0 methylglucuronic acid branching. In addition, arabinose branching as well as acetylation may be present. Gymnosperms contain glucomannans comprised primarily of D-mannosyl and D-glucosyl residues.
A mixture of glycolytic enzymes containing xylanase, mananase and other activities
from Aspergillus niger
Product Number H2125
Xylanase catalyzes the endohydrolysis of 1,4-β-D-xylosidic linkages in xylans yielding various 1,4-β-D-xylooligosaccharides
Pectins are complex branched heteropolysaccharides primarily containing an α-(1-4) polygalacturonic acid backbone which can be randomly acetylated and methylated. Three different pectins have been isolated from plant cell walls.
Homogalacturonan, Poly-α-(1,4)-Galacturonic acid backbone with random partial methylation and acetylation
Rhamnogalacturonan I, Alternating α-(1,2)-L-rhamnosyl-α-(1,4)-Dgalacturonosyl backbone with two types of branching composed of a ribfuranose or galactose oligomers
Rhamnogalacturonan II, Poly-α-(1,4)-D-Galacturonic Acid backbone with random partial methylation, acetylation and four types of branching
Pectinase catalyzes the random hydrolysis of 1,4-α-D-galactosiduronic linkages in pectin and other galacturonans.
from Aspergillus aculeatus
Product Number P2611 (Novozymes Pectinex® Ultra SPL)
from Aspergillus niger
Product Number P4716 (aqueous glycerol solution, min. 5 un/mg protein)
from Rhizopus sp.
Product Number P2401 (Crude Powder 400-800 un/g)
EC# 184.108.40.206 & 220.127.116.11
Reported to contain two types of pectinase, endopolygalacturonase (EC18.104.22.168), endo-pectin lyase (EC22.214.171.124) and a maceration stimulating factor.
Pectolyase catalyzes the eliminative cleavage of (1-4)-α-D-galacturonan methyl ester to give oligosaccharides with 4-deoxy-6-O-methyl- α-D-galact-4-enuronosyl groups at their non-reducing ends. Pectinase catalyzes the random hydrolysis of 1,4-α-D-galactosiduronic linkages in pectin and other galacturonans.
Synonyms: pectin methylesterase
Pectinesterase catalyzes the hydrolysis of the methyl esters of pectin to yield pectate and methanol.
from orange peel
Product Number P5400 (lyophilized, 350-700 un/mg)
To some, lignin appears to be a foreign substance resembling a randomly modified polystyrene polymer, not resembling any of the more familiar biopolymers composed of carbohydrates, amino acids or nucleotides. However, next to cellulose, it is the second most abundant biopolymer on earth comprising as much as 30% of the dry mass of wood. Unlike other biopolymers, enzymatic degradation is oxidative vs. hydrolytic and is limited to a select group of fungi and bacteria. Lignins appear to be heterogeneous and random with regards to degree of polymerization, branching, and monomer composition and sequence.
Three basic monomers of lignin
Synonyms: ligninase, LiP
Catalyzes the oxidative cleavage of carbon-carbon and ether bonds in lignin-related compounds
Product Number 42603 >0.1 units/mg
Synonyms: Benzenediol:oxygen oxidoreductase
Enzyme Commission (EC) Number: 126.96.36.199
from Trametes versicolor
Product Number 38429 powder, light brown, ≥0.5 units/mg
from garicus bisporus
Product Number 40452 powder, deep brown, ≥4 units/mg
from Rhus vernificera
Product Number L2157 crude acetone powder, ≥50 units/mg solid
Synonyms: Manganese-dependent lignin peroxidase, MnP
A hemoprotein. Involved in the oxidative degradation of lignin in white rot basidiomycetes.
Product Number 93014 >20 units/G
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