BioFiles Volume 6, Number 5 — Centrifugation
The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify organelle fractions from gently homogenized samples. To be suitable for further analysis, isolated organelles must be relatively pure, and structurally and functionally intact. It is also important to verify the organelle integrity with robust assays.
We offer several organelle isolation and enrichment kits, companion organelle functionality assays, and antibodies that are suitable for evaluation of samples from plant, animal, and cell culture sources.
Antibodies
Application Abbreviation Table
Antibodies
Product Name | Host | Clone No. | Form | Gene Symbol |
---|---|---|---|---|
Anti-GM130 (C-terminal) | rabbit | — | affinity isolated antibody | Golga2, rat Golga2, mouse GOLGA2, human |
Anti-GRP78/BiP (ET-21) | rabbit | — | IgG fraction of antiserum | Hspa5, mouse HSPA5, human Hspa5, rat |
Anti-Nuclear Pore Complex Proteins | mouse | 414 | purified immunoglobulin | — |
Anti-PMP70 | rabbit | — | affinity isolated antibody | Abcd3, mouse ABCD3, human Abcd3, rat |
Species Reactivity | Application | Cat. No. |
---|---|---|
human mouse rat | IF (i) IP WB CL | G7295-200UL |
Xenopus chicken hamster human mouse rat | ICC WB | G9043-200UL |
Xenopus human mouse rat yeast | EM ICC IP WB | N8786-200UL |
human mouse rat | IF (i) IP WB CL | P0497-200UL |
Application Abbreviation Table | ||||
---|---|---|---|---|
Application | Abbreviation | Application | Abbreviation | |
ANA-indirect immunofluorescence | IF (ANA) | Immunohistochemistry | IHC | |
Capture ELISA | ELISA (c) | Immunohistochemistry (formalin-fixed, paraffin-embedded sections) | IHC (p) | |
Direct ELISA | ELISA (d) | |||
Direct immunofluorescence | IF (d) | Immunohistochemistry (frozen sections) | IHC (f) | |
Dot blot | DB | Immunoprecipitation | IP | |
Dot immunobinding | DIBA | Indirect ELISA | ELISA (i) | |
Electron microscopy | EM | Indirect immunofluorescence | IF (i) | |
Enzyme immunoassay | EIA | Microarray | ARR | |
Flow cytometry | FACS | Neutralization | Neutral | |
Immunoblotting | WB | Ouchterlony double diffusion | ODD | |
Immunoblotting (chemiluminescent) | WB CL | Particle immunofluorescence | PIFA | |
Immunocytochemistry | ICC | Quantitative precipitin assay | QPA | |
Immunoelectrophoresis | IEP | Radioimmunoassay | RIA |
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