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Merck

Organelle Isolation

Mark Frei

BioFiles Volume 6, Number 5 — Centrifugation

The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify organelle fractions from gently homogenized samples. To be suitable for further analysis, isolated organelles must be relatively pure, and structurally and functionally intact. It is also important to verify the organelle integrity with robust assays.

We offer several organelle isolation and enrichment kits, companion organelle functionality assays, and antibodies that are suitable for evaluation of samples from plant, animal, and cell culture sources.

Antibodies
Application Abbreviation Table

Antibodies

Product NameHostClone No.FormGene Symbol
Anti-GM130 (C-terminal)rabbitaffinity isolated antibodyGolga2, rat
Golga2, mouse
GOLGA2, human
Anti-GRP78/BiP (ET-21)rabbitIgG fraction of antiserumHspa5, mouse
HSPA5, human
Hspa5, rat
Anti-Nuclear Pore Complex
Proteins
mouse414purified immunoglobulin
Anti-PMP70rabbitaffinity isolated antibodyAbcd3, mouse
ABCD3, human
Abcd3, rat
Species ReactivityApplicationCat. No.
human
mouse
rat
IF (i)
IP
WB CL
G7295-200UL
Xenopus
chicken
hamster
human
mouse
rat
ICC
WB
G9043-200UL
Xenopus
human
mouse
rat
yeast
EM
ICC
IP
WB
N8786-200UL
human
mouse
rat
IF (i)
IP
WB CL
P0497-200UL

Application Abbreviation Table

ApplicationAbbreviation ApplicationAbbreviation
ANA-indirect immunofluorescenceIF (ANA)ImmunohistochemistryIHC
Capture ELISAELISA (c)Immunohistochemistry (formalin-fixed, paraffin-embedded sections)IHC (p)
Direct ELISAELISA (d)
Direct immunofluorescenceIF (d)Immunohistochemistry (frozen sections)IHC (f)
Dot blotDBImmunoprecipitationIP
Dot immunobindingDIBAIndirect ELISAELISA (i)
Electron microscopyEMIndirect immunofluorescenceIF (i)
Enzyme immunoassayEIAMicroarrayARR
Flow cytometryFACSNeutralizationNeutral
ImmunoblottingWBOuchterlony double diffusionODD
Immunoblotting (chemiluminescent)WB CLParticle immunofluorescencePIFA
ImmunocytochemistryICCQuantitative precipitin assayQPA
ImmunoelectrophoresisIEPRadioimmunoassayRIA

Materials
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