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MilliporeSigma

Antibody Support Reagents

DESIGNED TO COMPLETE YOUR ANTIBODY WORKFLOW

With the highest quality reagents and chemicals, we are the supplier of 90,000+ antibodies to the research community. We bring quality and diverse antibody support reagents for your antibody workflows. Whether you use our antibodies in their wide range of validated applications or purifying antibodies in your lab, we have the tools to support your research from start to finish.

Explore our offering of suitable world-class Antibody Support Tools

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ADJUVANTS

Adjuvants, when used in conjunction with an immunogen during inoculation, can elicit a more intense immune response or stimulate immune response to antigens that are not inherently immunogenic. Explore our offering of adjuvant reagents, including our Sigma Adjuvant System® oil, Freund’s Adjuvant, Hemocyanin, Maleimide Activated BSA, Pristane synthetic, and TiterMax® Adjuvant liquids.

Prestige PrEST Antigens™ AND BLOCKING PROTOCOL POWERED BY ATLAS ANTIBODIES

Strengthen your research with Prestige Antibodies® by complementing with corresponding Prestige PrEST Antigens™. Every Prestige antibody has its immunizing antigen available for use in blocking or as a control.

ANTIBODY BLOCKING REAGENTS AND SUBSTRATES

Incubation with blocking reagents prevents non-specific binding of your antibody of interest and background staining by blocking reactive epitopes and endogenous proteins. Ready-to-use blocking reagents are available for use in a variety of immunoassays like western blot or immunohistochemistry. We also offer a full range of substrates to use with your enzyme-conjugated secondary antibodies.

CELL CAPTURE IMAGING REAGENTS

If your workflows include low cell count samples or rare cells, such as CTCs, stem cells, or cells infected with viruses, then our Cell Capture Imaging Reagents (CCIR) will provide you better results. They provide a single vial solution to the extreme cell loss encountered in standard Immunocytochemistry (ICC), Immunofluorescence (IF), and cytospin protocols. The CCIR allows antibodies and other needed imaging reagents to pass through a permeable hydrogel thin film. Offers mechanical strength to withstand routine pipetting. The cells treated with CCIR can then be imaged through a single centrifugation step in any standard, unmodified microtiter plate.

Antibody conjugation and labeling kits for your immunorelated workflow needs

Figure 2.Antibody conjugation & labeling kits for your immunorelated workflow needs

ANTIBODY CONJUGATION AND LABELING KITS: AVIDIN, STREPTAVIDIN & BIOTIN CONJUGATES

The strong affinity interaction between avidin family glycoproteins and biotin can be a useful way to amplify signal detection. The avidin-biotin system has been used in immunohistology, immunocytology, immunoblotting, and ELISA.

When used in conjunction with a biotinylated secondary antibody, the complexes formed between biotin and conjugated avidin can increase sensitivity. Explore our offering of fluorophore, enzymes, and other labeling conjugates.

Visikol® Tissue Clearing Solutions, Kits and Protocols

Visikol® HISTO™ and HISTO-M™ tissue clearing reagents and kits offer an easy-to-use technique to clarify bioimaging in a way that is compatible with your immunolabeling applications and easily reversible.

Visikol® Tissue Clearing Method Features and Benefits include:

  • A solvent-based approach
  • Fast and less damaging to tissues
  • Reversible to allow validation against traditional histology

For 3D cell models, where thickness and opacity can make it difficult for light to penetrate, HISTO-M™ presents an immunofluorescence-compatible solution for complete 3D cell culture model characterization and more accurate drug screening.

Rat brain after clearing

Figure 1A.Reversible nature of HISTO tissue clearing method. Rat brain after the clearing process.

Tissue opacity restored after reversal

Figure 1B.Tissue opacity restored after reversal.

IMMUNOGLOBULIN CONTROLS, HUMAN SERUM CONTROLS, AND BOVINE SERUM ALBUMIN

Normal sera and purified immunoglobulin proteins can be used as blocking agents or as positive and negative controls in many immunoassay applications. Explore our offering of immunoglobulin proteins, human and mouse serum controls, bovine serum albumin and more commonly used species and antibody isotypes.

ANTIBODY PURIFICATION METHOD, PROTOCOL, AND KITS

Are you trying to generate an antibody in your laboratory?
Successful antibody production depends upon utilizing quality reagents at each important steps. We have everything you need to cover your workflow from inoculation to purification. Adjuvants and carrier proteins can improve immune response on the front end while our Protein A/G/L resins, adsorption resins, and antibody-agarose products address your purification needs.

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Related Applications
Gel Electrophoresis
Protein gel electrophoresis is used to separate proteins for purification, characterization, and detection. Methods such as native PAGE, SDS-PAGE, 2D PAGE, and isoelectric focusing (IEF) are used in preparation for downstream applications including Western blotting, mass spectrometry, and proteomic analysis.
Western Blotting
An overview of the principles and applications of Western blotting for protein detection. Includes links to detailed protocols, videos, and other resources for protein extraction, gel electrophoresis, transfer to PVDF or nitrocellulose membranes, and chemiluminescent, colorimetric, and fluorescent detection methods.
(ELISA) Enzyme-Linked Immunosorbent Assay
Enzyme-linked immunosorbent assay (ELISA) include direct, indirect, and capture (sandwich) detection formats for identifying and quantifying a specific antigen.
Immunohistochemistry
Immunohistochemistry (IHC) is a common immunostaining technique that is used by researchers to identify specific antigens of interest in healthy and diseased tissues.
Flow Cytometry
Flow cytometry is a powerful molecular and cellular biology tool that enables multi-parametric analysis of individual cells.
Protein Quantitation
Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.
Related Product Categories
ZooMAb® Antibodies
Experience ZooMAb® recombinant antibodies, engineered for exceptional consistency and performance in various applications.
Small Pack Size Antibodies
Antibodies in 25 μg or 25 μL small pack sizes for your immunocytochemistry (ICC), immunohistochemistry (IHC), immunofluorescence (IF), immunoprecipitation (IP), flow cytometry, ELISA and western blot applications.
Enhanced Validation Antibodies
Our enhanced validation antibodies are characterized using numerous molecular and cell-based techniques and assays to provided superior assurance with target specificity for your required application needs.
Protein Gel Electrophoresis and Transfer Equipment
Electrophoresis tanks, blotting systems, and power supplies for protein gel electrophoresis and wet and semi-dry transfer.
Immunodetection Reagents & Substrates
High quality blocking reagents, secondary antibodies, and HRP and AP substrates for Western blotting and immunoblotting detection.
Protein Electrophoresis Gels & Buffers
Precast polyacrylamide protein gels, running buffers, and hand-casting reagents for hand-pouring polyacrylamide gels for PAGE and SDS-PAGE protein gel electrophoresis.
Protein Electrophoresis Gel Stains
High sensitivity colorimetric and fluorescent protein gel stains, fixing solutions, and de-staining reagents for polyacrylamide gels, agarose gels, and PVDF and nitrocellulose membranes.
Protein Electrophoresis & Western Blotting
Browse our products for protein electrophoresis and Western blotting, including Immobilon transfer membranes, transfer buffers, electrophoresis equipment, gels, gel stains, protein markers, and immunodetection reagents.
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