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8.51027

Sigma-Aldrich

Biotin-ONp

Novabiochem®

Synonym(s):

Biotin-ONp, Biotin p-nitrophenyl ester,5-(2-Oxo-hexahydro-thieno[3,4-d]imidazol-6-yl)-pentanoic acid p-nitrophenyl ester

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25 UNITS
$335.00

$335.00


Estimated to ship onMarch 22, 2025


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25 UNITS
$335.00

About This Item

Empirical Formula (Hill Notation):
C16H19N3O5S
CAS Number:
Molecular Weight:
365.40
MDL number:
UNSPSC Code:
12352108
NACRES:
NA.22

$335.00


Estimated to ship onMarch 22, 2025


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Quality Level

product line

Novabiochem®

assay

≥97.0% (HPLC)
≥98% (TLC)

form

powder

reaction suitability

reaction type: Biotinylations

manufacturer/tradename

Novabiochem®

mp

163-165 °C

application(s)

peptide synthesis

storage temp.

15-25°C

SMILES string

S1[C@H]([C@H]3NC(=O)N[C@H]3C1)CCCCC(=O)Oc2ccc(cc2)[N+](=O)[O-]

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This Item
MABF1966MABF81MABF2042
clone

C305, monoclonal

clone

22H9, monoclonal

clone

6F3.1, monoclonal

clone

1A6, monoclonal

biological source

mouse

biological source

rat

biological source

mouse

biological source

mouse

antibody form

culture supernatant

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

UniProt accession no.

Q6P4I7

UniProt accession no.

Q8CII9

UniProt accession no.

P51449

UniProt accession no.

Q9HC73

technique(s)

immunoprecipitation (IP): suitable, multiplexing: suitable

technique(s)

flow cytometry: suitable

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

flow cytometry: suitable

General description

Pre-activated reagent for the introduction of biotin by solid phase synthesis. More soluble and more reactive than 851023 [1]. This reagents has recently been found to be effective in SPOT synthesis of biotinylated peptides [2].

Associated Protocols and Technical Articles
Biotinylation Reagents for Peptide Synthesis

Automate your Biotin tagging with Synple Automated Synthesis Platform (SYNPLE-SC002)

Literature references

[1] B. Baumeister, et al. Int. J. Pept. Res. Ther., 11, 139.
[2] D. Winkler & P. McGeer (2008) Proteomics, 8, 961.

application

  • Mechanistic studies of a cell-permeant peptide designed to enhance myosin light chain phosphorylation: This study discusses the synthesis and applications of cell-permeant peptides involving biotin-ONP for enhanced cellular processes (Almansour, 2017).
  • Structure-function analysis of tight junction-directed permeation enhancer PIP250: Provides insights into the utility of biotin-ONP in analyzing the structure and function of peptides used to enhance cellular permeation (Taverner et al., 2023).

Linkage

Replaces: 01-63-0116

Analysis Note

Color (visual): white to slight yellow to beige
Appearance of substance (visual): powder
Identity (IR): passes test
Optical rotation α 25/D (c=1 in dioxan): +34.5 - +39.5 °
Purity (TLC(0811)): ≥ 98 %
Assay (HPLC, area%): ≥ 97.0 % (a/a)

To see the solvent systems used for TLC of Novabiochem® products please click here.

Legal Information

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Articles

Biotin-labelled peptides find applications in immunology and histochemistry for affinity purification and receptor localization.

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