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MilliporeSigma

U-87 MG Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 89081402, human brain (glioblastoma astrocytoma), Epithelial-like

Synonym(s):

U-87MG Cells, U87 Cells, U87MG Cells

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About This Item

UNSPSC Code:
41106514

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Product Name

U-87 MG Cell Line human, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 89081402, human brain (glioblastoma astrocytoma), Epithelial-like

biological source

human brain (glioblastoma astrocytoma)

packaging

tube of 5 μg 89081402-DNA-5UG
pkg of vial of cells 89081402-1VL

growth mode

Adherent

karyotype

2n = 46

morphology

Epithelial-like

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Application

Neural studies
U-87 MG cell line has been used to study the effects of the lysosomal destabilizing drug Siramesine on glioblastoma. It has also been used to study calcium-independent disruption of microtubule dynamics by nanosecond pulsed electric fields.

Biochem/physiol Actions

Derived from a malignant glioma from a female patient by explant technique. It is reported to produce a malignant tumour consistent with glioblastoma in nude mice.
Human glioblastoma astrocytoma
STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 8,11
D16S539: 12
D5S818: 11,12
D7S820: 8,9
THO1: 9.3
TPOX: 8
vWA: 15,17

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Preparation Note

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

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