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MilliporeSigma

F4523

Fast Red TR/Naphthol AS-MX

SIGMAFAST™, fluorogenic, tablet

Synonym(s):

4-Chloro-2-methylbenzenediazonium / 3-Hydroxy-2-naphthoic acid 2,4-dimethylanilide phosphate tablets

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.83
MDL number:
MFCD00132253

Key Documents

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Product Name

SIGMAFAST™ Fast Red TR/Naphthol AS-MX Tablets, tablet, To prepare 10 mL

form

tablet

storage temp.

−20°C

Quality Level

200

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Application

Developed for use as a precipitating substrate for the detection of alkaline phosphatase activity in immunochemistry. Primary uses are in immunohistology, immunoblotting, or dot blotting. SIGMAFAST™ Fast Red TR/Naphthol AS-MX tablets contain 0.6 mM levamisole to block endogenous alkaline phosphatase activity. SIGMAFAST™ Fast Red TR/Naphthol AS-MX Tablets require no additional steps or buffers to prepare an active substrate solution.
SIGMAFAST™ Fast Red TR/Naphthol AS-MX tablets has been used in fluorescent in situ hybridization for the analysis of gene expression in neurons and synapses.

Preparation Note

Each tablet set dissolved in 10 mL of deionized water yields a ready-to-use buffered solution.

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

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Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Sol. 2 - Skin Irrit. 2

Storage Class

4.1B - Flammable solid hazardous materials

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Certificates of Analysis (COA)

Lot/Batch Number
0000387761
0000387761
SLCN0642
SLCF5842
SLBR9488V

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Calcium imaging of basal forebrain activity during innate and learned behaviors
Harrison TC, et al.
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Nature, 545(7655), 477-481 (2017-05-18)
In humans and other mammalian species, lesions in the preoptic area of the hypothalamus cause profound sleep impairment, indicating a crucial role of the preoptic area in sleep generation. However, the underlying circuit mechanism remains poorly understood. Electrophysiological recordings and
G B Baretton et al.
British journal of cancer, 80(3-4), 546-555 (1999-07-17)
The molecular mechanisms leading to androgen-independent growth in prostate cancer (PC) are poorly understood. Androgen deprivation therapy (ADT) results physiologically in a decrease in proliferation and an increase in programmed cell death (PCD)/apoptosis. The aim of our study was to
Ulla R M Holopainen et al.
Journal of the science of food and agriculture, 92(15), 2944-2951 (2012-05-03)
Hordeins are major storage proteins of barley (Hordeum vulgare L.) grains and are considered to influence malting and brewing by forming a matrix surrounding the starch granules which affects the release of fermentable sugars. However, the extent to which environmental
K Buchmann
Folia parasitologica, 45(4), 312-318 (1998-12-30)
Histochemical studies of enzyme activities and structural elements in Gyrodactylus derjavini Mikailov, 1975 parasitizing fins of Oncorhynchus mykiss Walbaum were conducted. Marked activities of non-specific esterase, acid phosphatase, alkaline phosphatase and amino-peptidase were found in the intestinal caeca of the
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