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P3201

Platelet-Derived Growth Factor-BB human

≥98% (HPLC and SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym(s):

PDGF-BB

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.77
MDL number:
MFCD00213023
Biological source:
human
Recombinant:
expressed in E. coli
Assay:
≥98% (HPLC and SDS-PAGE)
Form:
lyophilized powder
Mol wt:
~25 kDa
Impurities:
≤1 EU/μg

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Product Name

Platelet-Derived Growth Factor-BB human, PDGF-BB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

biological source

human

recombinant

expressed in E. coli

assay

≥98% (HPLC and SDS-PAGE)

form

lyophilized powder

potency

1.0-3.0 ng/mL EC50

quality

endotoxin tested

mol wt

~25 kDa

packaging

pkg of 10 μg, pkg of 50 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤1 EU/μg

UniProt accession no.

P01127

storage temp.

−20°C

Gene Information

human ... PDGFB(5155)

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General description

Platelet-derived growth factor (PDGF) is expressed as homo and heterodimers : PDGF-AB FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD. The PDGF gene is highly conserved mapped to human chromosome 7p22.3. The active form of Pdgf exists as disulfide-linked dimers. It consists of cystine-knot-fold growth factor domain, which is highly conserved, except for the N-terminal prodomain, which shows variation in length among the isoforms.

Application

Platelet-Derived Growth Factor-BB human has been used :
  • As a growth supplement in proximal tubule and glomerular explant cell culture
  • In three-dimensional scaffold preparation
  • To study the effect of brivanib on growth factor signaling pathways
  • In transwell migration assay

Biochem/physiol Actions

Platelet-derived growth factor (PDGF) elicits multifunctional actions with a variety of cells. It is mitogenic to mesoderm-derived cells, such as dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells, and chondrocytes. PDGF significantly activates chemotaxis of neutrophils, monocytes, and fibroblasts. It increases the synthesis of phospholipids, cholesterol esters, glycogen and prostaglandins, and modulates LDL (low density lipoprotein) receptor binding.
PDGF stimulates fibroblast proliferation and extracellular matrix production in order to facilitate tissue repair. PDGF-BB isomer is found to be critical in angiogenesis and is highly expressed in tumor, mediating oncogenic pericyte recruitment. PDGF-BB is an important regulator of bone homeostasis, repair and regeneration.

Physical form

Lyophilized from a 0.2 μm filtered solution in 30 mM acetic acid.

Analysis Note

The biological activity is measured by its ability to stimulate 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts.

Other Notes

Dimer of the B chain of human PDGF.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000478729
0000478729
0000513041
0000513041
0000482676

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Injury induces in vivo expression of platelet-derived growth factor (PDGF) and PDGF receptor mRNAs in skin epithelial cells and PDGF mRNA in connective tissue fibroblasts
Antoniades HN
Proceedings of the National Academy of Sciences of the USA, 88.2, 565-569 (1991)
Rare incorporation of bone marrow-derived cells into kidney after folic acid-induced injury.
Szczypka MS, et al.
Stem Cells, 23.1, 44-54 (2005)
Brivanib attenuates hepatic fibrosis in vivo and stellate cell activation in vitro by inhibition of FGF, VEGF and PDGF signaling.
Nakamura I, et al.
PLoS ONE, 9.4 (2014)
Clinical Findings Associated with a De Novo Partial Trisomy 10p11. 22p15. 3 and Monosomy 7p22. 3 Detected by Chromosomal Microarray Analysis.
Kohannim O,et al.
Case Reports in Genetics, 2011 , 1-6 (2011)
Yasutoshi Takashima et al.
American journal of physiology. Renal physiology, 310(5), F395-F408 (2016-01-15)
Glomerular integrity and functions are maintained by growth factor signaling. Heparan sulfate, the major component of glomerular extracellular matrixes, modulates growth factor signaling, but its roles in glomerular homeostasis are unknown. We investigated the roles of heparan sulfate 6-O-endosulfatases, sulfatase
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Articles

Platelet Derived Growth Factors (PDGF)

Platelet-Derived Growth Factor is the principal mitogen found in mammalian serum and is released from platelets during clot formation.

Protocols

Guide: Expansion & Differentiation of Human Mesenchymal

TrueGel3D® Hydrogel Plate protocol guides high-throughput culture of human adipose MSCs for screening applications.

Related Content

Data Sheet - P3201

Instructions

Global Trade Item Number

SKUGTIN
P3201-10UG04061834367475
P3201-50UG04061834367482

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