30-1150
TriAltus™
TriAltus™ SUMO Protease
100 mg, Yeast protein overexpressed in E. coli with histidine (His) tag
Synonym(s):
SUMO, SUMO Protease, SUMO P, Ulp, Ulp1, Enzyme, SUMO Enzyme, Protease
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General description
SUMO Protease can be used for cleaving both natural SUMO-modified proteins and recombinant SUMO fusion proteins. This powerful site-specific protease targets the tertiary structure of the Ubiquitin-like SUMO domain. It hydrolyzes the peptide bond in the x–Gly–Gly–x sequence after the di-glycine bond, ensuring precise cleavage without leaving any residual amino acids.
SPECIFICATIONS
PURITY | ~100% (SDS gel analysis)
ENZYME SOURCE | Yeast protein overexpressed in E. coli with histidine (His) tag
CLEAVAGE SITE | SUMO Protease cleaves after the C-terminal glycine at the end of the SUMO sequence. If the target protein is fused downstream of the SUMO C-terminus, SUMO Protease cleavage renders the native protein sequence without any additional amino acids.
CLEAVAGE EFFICIENCY | 1 µg protease will digest 2 mg protein with 99% efficiency at 4 °C in 40 minutes.
STORAGE/SHIPPING CONCENTRATION | ~7.5 mg/mL
SHIPPING CONDITIONS | We ship SUMO protease on dry ice in buffer (15 mM Tris (pH 8.0), 0.2 M NaCl, 50% glycerol).
RECOMMENDED STORAGE CONDITIONS | -80°C long term or -20°C for 2-3 months in shipping buffer
EXPIRATION | 6 months from receipt when stored as directed
SKU |
1 mg: 30-1130
5 mg: 30-1135
10 mg: 30-1140
50 mg: 30-1146
100 mg: 30-1150
Additional processing information: This highly efficient SUMO protease is compatible with TriAltus™ CLiM protein affinity purification technology.
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