30-2150
TriAltus™
TriAltus™ PreScission Protease
100 mg, Human Rhinovirus (HRV) 3C Protease overexpressed in E. coli with a glutathione S-transferase (GST) tag
Synonym(s):
PreScission, PreScission Protease, PSC, PSC P, Enzyme, Precission Enzyme, Protease
About This Item
General description
The PreScission protease (PSC) is a human rhinovirus (HRV) type 14 3C protease fused to glutathione S-transferase (GST). This protease recognizes and cleaves the site specific sequence LEVLFQ/GP (where “/” indicates the cleavage site).
SPECIFICATIONS
PURITY | >99% (SDS gel analysis)
ENZYME SOURCE | Human Rhinovirus (HRV) 3C Protease overexpressed in E. coli with a glutathione S-transferase (GST) tag
CLEAVAGE SITE | PSC recognizes the sequence LEVLFQGP and cleaves between the glutamine (Q) and glycine (G)
CLEAVAGE EFFICIENCY | 20 µg protease will digest 2 mg protein with 99% efficiency at 4°C in 60 minutes.
STORAGE/SHIPPING CONCENTRATION | ~5 mg/mL
SHIPPING CONDITION | We ship PreScission Protease on dry ice in buffer (20 mM Tris (pH 8.0), 0.1 M NaCl, 1 mM EDTA, 50% glycerol, 1 mM βMe).
RECOMMENDED STORAGE CONDITIONS | -80 °C long term or -20 °C for 2-3 months in shipping buffer
EXPIRATION | 6 months from receipt when stored as directed
SKU |
1 mg: 30-2130
5 mg: 30-2135
10 mg: 30-2140
50 mg: 30-2146
100 mg: 30-2150
Additional processing information: This highly efficient PreScission protease is compatible with TriAltus′ CLiM protein affinity purification technology.
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