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Protein Purification Chromatography

Magnetic beads for protein purification

For decades, liquid chromatography has been a powerful tool for isolating proteins, peptides, and other molecules from complex mixtures. Protein A, Protein G and Protein L bind IgG from a variety of species; resins crosslinked to these proteins are used to purify monoclonal or polyclonal antibodies. Affinity resins for fusion tags added to recombinant proteins allow purification by affinity chromatography. To support your protein research, we offer a broad range of protein chromatography tools for antibody purification and affinity purification, including the FLAG® system of recombinant fusion protein detection and purification.  

The image captures a precise moment in a laboratory setting, where a blue pipette is dispensing a single clear liquid droplet into a small clear container.

FLAG purification

Expression vectors, affinity resins, and antibodies for expressing, purifying, and detecting FLAG® tagged fusion proteins.

Recombinant/Fusion Tag Protein Purification

Affinity purification resins and buffers for purifying His-tagged, GST-tagged, FLAG® tagged, S-tag™, Strep-Tag II, and T7-tagged proteins under native or denaturing conditions using batch or column purification techniques.

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The image depicts two HiTrap Protein G HP syringes used for protein purification. One syringe has a capacity of 5 ml and the other 1 ml. Both have a white body, red caps, and black plungers, and are set against a white background. The larger syringe is labeled “HiTrap™ 5 ml Protein G HP” and the smaller one “HiTrap™ 1 ml Protein G HP”.
Antibody Protein Purification

Antibody purification resins and prepacked columns for lab scale, process scale, and industry scale workflows.

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The image features a small white bottle with medication and three HiTrap Protein G HP syringes used for protein purification. The syringes have a white body, red caps, and black plungers. The bottle and syringes have labels with dosage instructions.
Biotinylated Protein Purification

Streptavidin and avidin resins and prepacked columns for small- to large-scale purification of biotinylated proteins and antibodies.

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