HPLC Analysis of Warfarin™ Anticoagulant on Ascentis® C18
Conditions | |
|---|---|
| Column | Ascentis C18, 15 cm x 4.6 mm I.D., 5 µm particles (581324-U) |
| Column Temp. | 35 °C |
| Mobile phase | [A] 20 mM phosphoric acid (pH 2.0, unadjusted): [B] methanol; (30:70, A:B) |
| Flow rate | 1 mL/min |
| Sample | 50 µg/mL each in mobile phase |
| Injection | 10 µL |
| Detector | UV, 280 nm |
Description | |
|---|---|
| Categories | Analytical Chromatography, Pharmaceutical Analysis, Anticoagulants |
| Featured Industry | Clinical Pharmaceutical (small molecule) |
| Legal Information | Ascentis is a registered trademark of Sigma-Aldrich Co. LLC, Warfarin is a trademark of Wisconsin Alumni Research Foundation |
| Suitability | Application for HPLC |
Materials
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