L1006

L-Lactic Dehydrogenase from bovine heart

Type XVII, buffered aqueous glycerol solution, ≥400 units/mg protein

• Synonym(s): Lactate, (S)-Lactate: NAD⁺ oxidoreductase, L-LDH, LAD, LD
• CAS Number: 9001-60-9
• Enzyme Commission number: 1.1.1.27 (BRENDA, IUBMB)
• EC Number: 232-617-8
• MDL number: MFCD00131466
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: bovine heart
• Quality Level: 200
• type: Type XVII
• Assay: 5.0—15.0 mg protein/mL (biuret)
• form: buffered aqueous glycerol solution
• specific activity: ≥400 units/mg protein
• mol wt: 140 kDa
• manufacturer/tradename: Sigma-Aldrich
• technique(s): cell based assay: suitable
• impurities: <10 μg/mg protein free ammonium

Description

General description

Research area: Cell signaling. L-LacticDehydrogenase (LDH) is present in the cell cytoplasm. and is part of the glycolytic pathway. Lactate dehydrogenase (LDH) is a ubiquitous molecule found in plants, yeast, mammals, and microorganisms and is a member of the oxidoreductase family.[1] It has five isozymes (LD₁ to LD₅) and this composition varies in different tissues. LD₁ is present at higher concentrations in the heart, kidneys, and erythrocytes. LD₅ is found in the liver and skeletal muscles.(2)

Application

L-Lactic Dehydrogenase from bovine heart has been used in ATPase assay of R2 complex Rvb1p-Rvb2p,[2] RecA protein[3] and sarcoplasmic reticulum Ca²⁺-ATPase (SERCA).[4]

Biochem/physiol Actions

Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.

Variation in the levels of L-Lactic Dehydrogenase (LDH) isoform is a diagnostic marker for tissue damage. The levels of LDH is useful in detecting dairy cattle disease, Mastitis.[5] Dye based purified LDH from bovine heart has analytical applications.[6]Lactic dehydrogenase (LDH)plays an essential role in the glycolytic pathway where it is involved in the conversionof pyruvate to lactate using NAD⁺as a co-factor. It is considered an important molecule that can be used incancer therapy as it acts as a glycolytic inhibitor. Inhibition of LDH isassociated with blocking of aerobic glycolysis in the tumour cells.[7]

Unit Definition

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

Physical form

Solution in 50% glycerol containing 0.025 M potassium phosphate buffer, pH 7.5

Analysis Note

Protein determined by biuret

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1