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L1006

Sigma-Aldrich

L-Lactic Dehydrogenase from bovine heart

Type XVII, buffered aqueous glycerol solution, ≥400 units/mg protein

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Synonym(s):
Lactate, (S)-Lactate: NAD+ oxidoreductase, L-LDH, LAD, LD
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

type

Type XVII

Quality Level

form

buffered aqueous glycerol solution

specific activity

≥400 units/mg protein

mol wt

140 kDa

cation traces

NH4+: ≤10 μg/mg protein

UniProt accession no.

foreign activity

glutamic-pyruvic transaminase and glutamic-oxalacetic transaminase ≤0.02%
pyruvate kinase, myokinase and α-glycerophosphate dehydrogenase ≤0.01%

storage temp.

2-8°C

Gene Information

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This Item
L2625L3916L2500
Gene Information

cow ... LDHA(281274), LDHB(281275)

Gene Information

cow ... LDHA(281274), LDHB(281275)

Gene Information

-

Gene Information

-

form

buffered aqueous glycerol solution

form

ammonium sulfate suspension

form

solution

form

ammonium sulfate suspension

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

UniProt accession no.

P19858, Q5E9B1

UniProt accession no.

P19858, Q5E9B1

UniProt accession no.

-

UniProt accession no.

-

mol wt

140 kDa

mol wt

-

mol wt

-

mol wt

140 kDa

General description

L-Lactic Dehydrogenase (LDH) is present in the cell cytoplasm and is part of the glycolytic pathway. It exists in different isoforms in heart and muscles. LDH is a tetramer corresponding to a molecular weight of 140 kDa. LDH has a nucleotide-cofactor binding site and has the structure of Rossmann fold.

Application

L-Lactic Dehydrogenase from bovine heart has been used in ATPase assay of R2 complex Rvb1p-Rvb2p, RecA protein and sarcoplasmic reticulum Ca2+-ATPase (SERCA).

Biochem/physiol Actions

Variation in the levels of L-Lactic Dehydrogenase (LDH) isoform is a diagnostic marker for tissue damage. The levels of LDH is useful in detecting dairy cattle disease, Mastitis. Dye based purified LDH from bovine heart has analytical applications.
Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.

Unit Definition

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

Physical form

Solution in 50% glycerol containing 0.025 M potassium phosphate buffer, pH 7.5

Analysis Note

Protein determined by biuret

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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25G
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Simultaneous purification of L-malate dehydrogenase and L-lactate dehydrogenase from bovine heart by biomimetic-dye affinity chromatography
Labrou NE and Clonis YD
Bioprocess and Biosystems Engineering, 16(3), 157-161 (1997)
Determination of lactate dehydrogenase (LDH) activity in milk by a fluorometric assay
Larsen T
The Journal of Dairy Research, 72(2), 209-216 (2005)
Purified E255L mutant SERCA1a and purified PfATP6 are sensitive to SERCA-type inhibitors but insensitive to artemisinins
Cardi D, et al.
The Journal of Biological Chemistry, 285(34), 26406-26416 (2010)
Active displacement of RecA filaments by UvrD translocase activity
Petrova V, et al.
Nucleic Acids Research, 43(8), 4133-4149 (2015)
Ligand binding and protein dynamics in lactate dehydrogenase
Pineda JR, et al.
Biophysical Journal, 93(5), 1474-1483 (2007)

Protocols

This procedure applies to all products from heart muscle that have a specification for L-Lactic Dehydrogenase activity.

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