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L3888

Sigma-Aldrich

D-Lactic Dehydrogenase from Lactobacillus leichmannii

lyophilized powder, 150-500 units/mg protein

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Synonym(s):
(R)-Lactate:NAD+ oxidoreductase, D-LDH
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

biological source

bacterial (Lactobacillus leichmannii)

Quality Level

form

lyophilized powder

specific activity

150-500 units/mg protein

composition

Protein, ~50% biuret

foreign activity

Malic dehydrogenase <0.5% of base activity

storage temp.

−20°C

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This Item
L9636L2011H9408
specific activity

150-500 units/mg protein

specific activity

≥80 units/mg solid

specific activity

≥250 units/mg protein (biuret)

specific activity

≥200 units/mg protein

form

lyophilized powder

form

lyophilized powder

form

ammonium sulfate suspension

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

-

Quality Level

200

Quality Level

200

foreign activity

Malic dehydrogenase <0.5% of base activity

foreign activity

-

foreign activity

Malic dehydrogenase <0.5% of base activity

foreign activity

lactic dehydrogenase ≤0.05%, malic dehydrogenase ≤0.1%

Application

In the food industry, the primary catalysis is coupled to conversion of NADH and H+ to NAD+ with diaphorase coupled with converting the non-fluorescent resazurin to the highly fluorescent substance resorufin to measure the content of D-lactate in food products.

Biochem/physiol Actions

D-lactic dehydrogenase catalyzes the conversion of pyruvate into D-lactate, with oxidation of NADH to NAD+. D-lactic dehydrogenase can also catalyze the reverse reaction, conversion of D-lactate into pyruvate with reduction of NAD+ to NADH.

Unit Definition

D-lactic dehydrogenase catalyzes the conversion of pyruvate into D-lactate, with oxidation of NADH to NAD+. D-lactic dehydrogenase can also catalyze the reverse reaction, conversion of D-lactate into pyruvate with reduction of NAD+ to NADH.
One unit will reduce 1.0 μmole of pyruvate to D-lactate per min at pH 7.0 at 25 °C.

Physical form

Lyophilized powder containing phosphate buffer salts

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Gerrit Stuivenberg et al.
Journal of food science and technology, 59(9), 3419-3427 (2022-07-26)
Recent studies suggest histamine and d-lactate may negatively impact host health. As excess histamine is deleterious to the host, the identification of bacterial producers has contributed to concerns over the consumption of probiotics or live microorganisms in fermented food items.
Andreas Neuner et al.
Biotechnology journal, 6(3), 318-329 (2011-03-04)
The Corynebacterium glutamicum ATCC 13032 lysC(fbr) strain was engineered to grow fast on racemic mixtures of lactate and to secrete lysine during growth on lactate as well as on mixtures of lactate and glucose. The wild-type C. glutamicum only grows
Martin Engqvist et al.
The Journal of biological chemistry, 284(37), 25026-25037 (2009-07-10)
The Arabidopsis thaliana locus At5g06580 encodes an ortholog to Saccharomyces cerevisiae d-lactate dehydrogenase (AtD-LDH). The recombinant protein is a homodimer of 59-kDa subunits with one FAD per monomer. A substrate screen indicated that AtD-LDH catalyzes the oxidation of d- and
Arnaud Mourier et al.
Biochimica et biophysica acta, 1777(10), 1283-1288 (2008-07-22)
Aerobically grown yeast cells express mitochondrial lactate dehydrogenases that localize to the mitochondrial inner membrane. The D-lactate dehydrogenase is a zinc-flavoprotein with high acceptor specificity for cytochrome c, that catalyzes the oxidation of D-lactate into pyruvate. In this paper, we
Takenori Shibahara et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 67(Pt 11), 1425-1427 (2011-11-22)
A dye-linked D-lactate dehydrogenase from the aerobic hyperthermophilic archaeon Aeropyrum pernix was crystallized using the hanging-drop vapour-diffusion method with polyethylene glycol 8000 as the precipitant. The crystals belonged to the monoclinic space group P2(1), with unit-cell parameters a = 63.4

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