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L3916

Sigma-Aldrich

L-Lactic Dehydrogenase from bovine heart

1000 units/mL

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Synonym(s):
Lactate, (S)-Lactate: NAD+ oxidoreductase, L-LDH, LAD, LD
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

form

solution

Quality Level

specific activity

1000 units/mL

storage temp.

2-8°C

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This Item
L2625L1378427211
form

solution

form

ammonium sulfate suspension

form

ammonium sulfate suspension

form

lyophilized

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

General description

LDH (lactic dehydrogenase) is a glycolytic enzyme. It is found in skeletal muscle, heart, liver, kidneys, brain, lungs and red blood cells. It comprises of five isoenzyme forms. LDH possess a tetrameric structure.

Application

L-Lactic Dehydrogenase from bovine heart has been used:
  • as a reference standard to measure the LDH (lactic dehydrogenase)
  • to quench protein-bound NADH in cells
  • to measure the lactate released by the cells in suspension

For use in enzymatic determination of lactate or pyruvate.

Biochem/physiol Actions

LDH (lactic dehydrogenase) helps in the reversible conversion of lactate to pyruvate.
Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.

Unit Definition

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

Physical form

Suspension in 2.2 M ammonium sulfate

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Articles

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Protocols

This procedure applies to all products from heart muscle that have a specification for L-Lactic Dehydrogenase activity.

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