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59747

Sigma-Aldrich

Lactic Dehydrogenase, recombinant from E. coli

≥90 U/mg

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Synonym(s):
(S)-Lactate: NAD+ oxidoreductase, L-Lactate Dehydrogenase, Lactate
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

recombinant

expressed in E. coli

Quality Level

form

powder

specific activity

≥90 U/mg

storage temp.

−20°C

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General description

LDH (lactic dehydrogenase), a glycolytic enzyme, particularly present in skeletal muscle, heart, liver, kidneys, brain, lungs and red blood cells. It has five isoenzyme forms. LDH possess a tetrameric structure.

Application

Lactic Dehydrogenase, recombinant from E. coli has been used:
  • in lactate dehydrogenase (LDH) and malate dehydrogenase 1 (MDH1)assays and cross-linking assays
  • to prepare assay buffer to measure pyruvate kinase (PYK) by coupled assay
  • in in vitro DltC D-alanylation assay

Biochem/physiol Actions

Conversion of L-lactate into L-pyruvate is crucial in hypoxic and anaerobic conditions, especially when synthesis of adenosine triphosphate (ATP) by oxidative phosphorylation is interrupted.
L-lactic dehydrogenase catalyzes the conversion of L-lactate into L-pyruvate while reducing NAD+ to NADH and H+.

Unit Definition

One unit corresponds to the amount of enzyme which reduces 1 μmol pyruvate per minute at pH 7.4 and 25°C (NADH as cofactor)

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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