03-32-5032

MMP-2/MMP-7 Substrate, Fluorogenic

Extremely sensitive fluorogenic substrate for matrix metalloproteinases (MMPs).

• 別名: MMP-2/MMP-7 Substrate, Fluorogenic
• 実験式（ヒル表記法）: C₄₉H₆₈N₁₄O₁₅
• 分子量: 1093.15
• UNSPSCコード: 12352209
• NACRES: NA.83

特性

• 品質水準: 200
• アッセイ: ≥95% (HPLC)
• 形状: solid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; desiccated
• 色: yellow
• 溶解性: 20% acetic acid: 1 mg/mL
• 蛍光検出: λ_ex ~325 nm; λ_em ~393 nm
• 輸送温度: ambient
• 保管温度: −20°C

詳細

詳細

Extremely sensitive fluorogenic substrate for matrix metalloproteinases (MMPs). Suitable for assessment of total activity in crude preparations by continuous assays (k_cat/K_m = 1.7 x 10⁵ M^-1sec^-1 for MMP-7 and 6.3 x 10⁵ M^-1sec^-1 for MMP-2).

生物化学的／生理学的作用

Cell permeable: no

Primary Target
Fluorogenic substrate for matrix metalloproteinases (MMPs)

Product does not compete with ATP.

Reversible: no

kcat/Km = 1.7 x 105 M-1sec-1 for MMP-7 and 6.3 x 105 M⁻¹ sec⁻¹ for MMP-2

警告

Toxicity: Standard Handling (A)

シーケンス

MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH₂ ·TFA [Dpa = N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]

物理的形状

Supplied as a trifluoroacetate salt.

再構成

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

その他情報

Knight, C.G., et al. 1992. FEBS Lett. 296, 263.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable