05-1517

Anti-hnRNP Q Antibody, clone 18E4

clone 18E4, from mouse

• 別名: heterogeneous nuclear ribonucleoprotein Q-like, Synaptotagmin-binding, cytoplasmic RNA-interacting protein, Glycine- and tyrosine-rich RNA-binding protein, GRY-RBP, NS1-associated protein 1
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• クローン: 18E4, monoclonal
• application: ICC; IP; WB
• 化学種の反応性: human
• テクニック: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• citations: 6

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 18E4, monoclonal
• 化学種の反応性: human
• テクニック: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBIアクセッション番号: NP_001153145.1
• UniProtアクセッション番号: O60506
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... SYNCRIP(10492)

詳細

詳細

Heterogeneous nuclear ribonucleoprotein Q commoly refered to as hnRNP Q can be found as three different isoforms derived from alternative splicing of a single gene. This RNA binding protein is implicated in control of mRNA precursor splicing for the survival of motor neurons (SMN) protein, a gene. Loss-of-function mutations of SMN are linked to spinal muscular atrophy (SMA) a common neurodegenerative disease. It has been shown that hnRNP Q proteins interact with SMN, and this is interacton is required for efficient pre-mRNA splicing in vitro. Current data suggests that hnRNP Q is a splicing modulator of SMN.

特異性

This antibody recognizes hnRNP Q.

免疫原

Epitope: Unknown

Recombinant protein corresponding to human hnRNP Q.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Research Sub Category
RNA代謝及び結合タンパク質

Immunocytochemistry
Cited by independent researcher using a representative lot.
Immunoprecipitation
Cited by independent researcher using a representative lot.

Use Anti-hnRNP Q Antibody, clone 18E4 (Mouse Monoclonal Antibody) validated in WB, ICC, IP to detect hnRNP Q also known as heterogeneous nuclear ribonucleoprotein Q-like.

品質

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1 µg/ml of this antibody detected hnRNP Q on 10 µg of HeLa cell lysate.

ターゲットの説明

The calculated molecular weight is 70 kDa Clone 18E4 can recognizes four bands on the Western Blot of total HeLa cell lysate corresponding to molecular masses of ~80, ~70, ~60 and ~55 kDa hnRNP Q has three isoforms of ~70– 55 kDa The ~80 kDa reactive band was identified as hnRNP R, which migrates at ~80 kDa on SDS–PAGE. (Mourelatos., Z., et al., EMBO J., 20, 5443-5452 (2001).)

物理的形状

Protein G Purified

Format: Purified

Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
HeLa cell lysate

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable