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由来生物
mouse
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
7H3, monoclonal
化学種の反応性
human
メーカー／製品名
Upstate®
テクニック
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
アイソタイプ
IgG
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... HNF1A(6927)
詳細
TCF 1 (T cell factor 1) is a member of the TCF/LEF (lymphoid enhancer factor) family of transcription factors. Members of this family along with b catenin, are involved in mediating Wnt signaling by transactivating downstream genes. TCF 1 is closely related to LEF-1, both of which are transcription factors expressed in mice during T cell differentiation. They regulate the T cell receptor a enhancer in transfections assays. TCF 1 becomes transcriptionally active through interaction with b catenin. Also, numerous splice variants of TCF 1 have been identified. Overexpression of TCF-1-specific splice forms appears to correlate with the metastatic behavior of colorectal cancer cells and with overproduction of lymphoid tyrosine protein kinase p56(lck).
特異性
Recognizes multiple splice isoforms of TCF-1.
免疫原
Maltose binding protein (MBP) fusion protein corresponding to residues 102-269 of human TCF-1A splice variant. The epitope maps to a region just N-terminal to the High Mobility Group (HMG) box
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
Research Sub Category
転写因子
転写因子
Detect TCF-1 with Anti-TCF-1 Antibody, clone 7H3 (Mouse Monoclonal Antibody), that has been shown to work in ICC, IHC & WB.
品質
routinely evaluated by immunoblot on Jurkat RIPA cell lysate
ターゲットの説明
26 kDa, 32 kDa, 38 kDa, 42 kDa, and 48 kDa
関連事項
Replaces: 04-1159
物理的形状
Protein G Purified
Format: Purified
Protein G Purified immunoglobulin in Immunoaffinity Purified immunoglobulin in 0.2M Tris-glycine, pH 7.5, 0.15M NaCl and 0.05% sodium azide
保管および安定性
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
アナリシスノート
Control
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
05-510:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
M Van de Wetering et al.
Molecular and cellular biology, 16(3), 745-752 (1996-03-01)
Previously, we reported the isolation of cDNA clones representing four alternative splice forms of TCF-1, a T-cell-specific transcription factor. In the present study, Western blotting (immunoblotting) yielded a multitude of TCF-1 proteins ranging from 25-55 kDa, a pattern not simply
Haiyan Li et al.
American journal of physiology. Cell physiology, 299(2), C422-C430 (2010-07-29)
In our previous studies, roles of gap junction and vascular endothelial growth factor in the cross-talking of human bone marrow stromal cells (HBMSCs) and human umbilical vein endothelial cells (HUVECs) have been extensively studied. The present study focused on the
Weichun He et al.
Journal of the American Society of Nephrology : JASN, 23(2), 294-304 (2011-11-19)
A variety of chronic kidney diseases exhibit reactivation of Wnt/β-catenin signaling. In some tissues, β-catenin transcriptionally regulates matrix metalloproteinase-7 (MMP-7), but the association between MMP-7 and Wnt/β-catenin signaling in chronic kidney disease is unknown. Here, in mouse models of both
Weichun He et al.
The Journal of biological chemistry, 285(32), 24665-24675 (2010-06-04)
Plasminogen activator inhibitor-1 (PAI-1) is a multifunctional glycoprotein that plays a critical role in the pathogenesis of chronic kidney and cardiovascular diseases. Although transforming growth factor (TGF)-beta1 is a known inducer of PAI-1, how it controls PAI-1 expression remains enigmatic.
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