06-1283

Anti-acetyl-p53 (Lys320) Antibody

from rabbit, purified by affinity chromatography

• 別名: Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, p53 antigen, p53 transformation suppressor, p53 tumor suppressor, transformation-related protein 53, tumor protein p53
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• クローン: polyclonal
• application: WB
• 化学種の反応性: human
• テクニック: western blot: suitable
• citations: 9

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: human
• 化学種の反応性（ホモロジーによる予測）: bovine (based on 100% sequence homology), chimpanzee (based on 100% sequence homology)
• テクニック: western blot: suitable
• NCBIアクセッション番号: NP_000537
• UniProtアクセッション番号: P04637
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: acetylation (Lys320)
• 遺伝子情報: human ... TP53(7157)

詳細

詳細

p53 was discovered in 1979 as a cellular protein associating with the transforming protein of SV40 tumor virus. Since then, many different biochemical functions have been attributed to the 53 kD phosphoprotein. Experimental evidence has suggested that p53 acts as a negative regulator of cell growth in normal cells (Finlay, 1989). Thus, the inactivation or mutation of p53 may be an essential step in the development of malignancy (Lane and Benchmol, 1990). Wild-type p53 levels in normal cells and tissues were found to be very low. Mutant p53 polypeptide, however, is often found to be present at high concentrations in mammalian tumors and tumor cell lines. For example, in an immuno-histochemistry study 40% of human breast cancer showed elevated levels of mutant p53 in the cell nucleus. Mutations of the p53 protein have some characteristic features:
a) Most of them are missense point mutations giving rise to an altered protein function.
b) Many -but not all- mutant p53 proteins exhibit a common mutant structure, which can be recognized by monoclonal antibodies specific for p53 in the mutant conformation.

特異性

This antibody recognizes p53 acetylated at Lys320.

免疫原

Epitope: Acetyl Lys 320

KLH-conjugated linear peptide corresponding to p53 at Lys320.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Research Sub Category
転写因子

Use Anti-acetyl-p53 (Lys320) Antibody (Rabbit Polyclonal Antibody) validated in WB to detect acetyl-p53 (Lys320) also known as Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, p53 antigen.

Western Blot Analysis: 5 µg/mL antibody detected p53 on 10 µg of A549 cells treated with UV & TSA lysate.

Western Blot (SNAP ID) Analysis: 5 µg/mL antibody detected p53 on 10 µg of recombinant proteins.

品質

Evaluated by Western Blotting using A549 cells treated with UV & TSA.
Western Blotting Analysis: 5 µg/mL of a representative lot of this antibody detected p53 on 10 µg of A549 cells treated with UV & TSA lysate.

ターゲットの説明

~ 53 kDa observed. Western Blot of A549 cells treated with UV & TSA show bands at ~48 kDa and ~14 kDa which are likely breakdown products of p53 reported for UV treated cells. (Sadji-Ouatas, 2002)

関連事項

Replaces: 06-915

物理的形状

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
Recombinant proteins

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable