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Merck

1.02129

Supelco

Chromolith® RP-18 endcapped (monolithic) HPLC Columns

L × I.D. 100 mm x 4.6 mm HPLC Column

別名:

Chromolith® Performance RP-18 endcapped 100-4.6 HPLC column

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About This Item

UNSPSCコード:
41115709
NACRES:
SB.52
カラムL × 内径:
10 cm × 4.6 mm
マトリックス活性基:
C18 bonding phase
ポアサイズ:
13 nm mesoporosity
2 μm macroporosity
Matrix:
monolithic silica

製品名

Chromolith® RP-18 endcapped, L × I.D. 100 mm x 4.6 mm HPLC column

物質

PEEK column

品質水準

認証

suitable for USP L1

製品種目

Chromolith®

特徴

endcapped

包装

1 ea of

標識化の程度

18% Carbon loading

パラメーター

200 bar pressure
45 °C max. temp.

テクニック

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)

カラムL × 内径

10 cm × 4.6 mm

Matrix

monolithic silica

マトリックス活性基

C18 bonding phase

ポアサイズ

13 nm mesoporosity
2 μm macroporosity

動作pH範囲

2-7.5

分離法

reversed phase

保管温度

no temp limit

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詳細

Chromolith® columns are made of monolithic silica with a characteristic bimodal pore structure.

Chromolith® Performance RP-18 endcapped HPLC columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å) and the surface modification is octadecylsilane with full end-capping. Chromolith® Performance RP-18 endcapped columns fall under the USP L1 Classification. The column efficiency is comparable to a 5 μm particle size fully porous particulate column.

Chromolith® Performance RP-18 endcapped columns are perfectly suitable for LC-MS applications.
The column dimension of 100 mm length and 4.6 mm I.D. is the best choice for separations of complex samples on any HPLC System.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
  • Rapid separations at very low column back-pressure
  • High matrix tolerance, allowing time and cost savings with simplified workflows
  • Cost savings due to extended column lifetimes
  • Connection of columns, flow gradients and the use of mobile phases with higher viscosity


The recommended Guard-columns are:
Chromolith® RP-18 endcapped 5-4.6 guard cartridges (3 pieces) [1.51452] with the Chromolith® Guard Cartridge Holder 5 mm x 4.6 mm [1.52032] Or Chromolith® RP-18 endcapped 10-4.6 guard cartridges (3 pieces) [1.51451] with the Chromolith® Guard Cartridge Holder 10 mm x 4.6 mm [1.52033]

The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]

Need more efficiency?
Chromolith® HighResolution RP-18 endcapped 100-4.6 HPLC columns [1.52022] provide a column efficiency exceeding 140,000 plates/meter.

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

アプリケーション


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アナリシスノート

Appearance: conforms
Carbon content: 17,0 - 18,6 %
Pressure: ≤ 30 bar
stability testing: conforms
Performance (anthracene)
Theoretical Plates (N/m) (Anthracene): ≥ 80000
Capacity factor (Anthracene): 3,9 - 4,6
Tailing (USP): 0,90 - 1,60 %
Selectivity
separation factor (anthracene/progesterone): 1,95 - 2,35

法的情報

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

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保管分類コード

10-13 - German Storage Class 10 to 13


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資料

Chromolith® RP-18e columns optimize Oligo Standard 6 separation with varied flow rates and ion-pairing reagent evaluation.

LC-UV-MS workflow details teriparatide peptide mapping, including enzymatic digestion, separation conditions, and QTOF mass spectrometer identification.

Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.

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