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詳細
His•Bind® purification kit includes pre-tested buffers specifically designed for IDA-based His•Bind Resin, enabling convenient and rapid one-step purification of proteins by metal affinity chromatography. It uses the His•Tag®/His•Bind® technology, that leverages the affinity between neighboring histidine in the His•Tag sequence and immobilized metal ions (such as Ni2+ or Co2+).
アプリケーション
His•Bind® Purification Kit has been used for: the purification of N-terminal 6-histidine-tagged aldehyde dehydrogenase isozyme, ALDH1A1 under nondenaturing conditions by nickel-NTA affinity chromatography, the purification of virus binding protein specific for Poliovirus Type 1, the purification of recombinant oE7, huhsp70 and oE7/huhsp70 proteins, to study the effective antitumor efficacy of human hsp70 and HPV16 oE7 fusion protein vaccine, the purification of recombinant exFPR1, MTBHsp70 and MTBHsp70-exFPR1 fusion proteins to study their role in cervical cancer immunotherapy
特徴および利点
- Includes His•Bind® Resin, His•Bind® buffer kit and chromatography columns.
- Solutions are included for Ni2+ charging, binding, washing, and elution.
- Columns are pre-packed with 1.25 ml bed volume of Ni2+-charged His•Bind Resin.
構成
•10 mlHis•Bind Resin
•1His•Bind Buffer Kit
•pkg/4Chromatography Columns
•1His•Bind Buffer Kit
•pkg/4Chromatography Columns
警告
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
その他情報
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
法的情報
HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
シグナルワード
Danger
危険有害性の分類
Aquatic Chronic 2 - Carc. 1A Inhalation - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1C - Skin Sens. 1 - STOT RE 1 - STOT RE 2 Inhalation
ターゲットの組織
respiratory tract irritation
保管分類コード
3 - Flammable liquids
引火点(°F)
86.0 °F
引火点(℃)
30 °C
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
消防法
第4類:引火性液体
第二石油類
危険等級III
非水溶性液体
Jan Code
70239-3:
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Human hsp70 and HPV16 oE7 fusion protein vaccine induces an effective antitumor efficacy
Oncology Reports, 30(1), 407-412 (2013)
MTBHsp70-exFPR1-pulsed Dendritic Cells Enhance the Immune Response against Cervical Cancer
Journal of Cancer, 10(25), 6364?6373-6364?6373 (2019)
A bioluminescence assay for aldehyde dehydrogenase activity
Analytical Biochemistry, 434(2) (2013)
Construction of a Cloning System for the Mass Production of a Virus-Binding Protein Specific for Poliovirus Type 1
Applied and Environmental Microbiology, 71(5) (2005)
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