ABD125

Anti-TFE3 Antibody

from rabbit, purified by affinity chromatography

• 別名: TFE3, Transcription factor E3, Class E basic helix-loop-helix protein 33, bHLHe33
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: human
• テクニック: immunocytochemistry: suitable; western blot: suitable
• NCBIアクセッション番号: NP_006512
• UniProtアクセッション番号: P19532
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... TFE3(7030)

詳細

詳細

The DNA-binding factor TFE3 contains adjacent helix-loop-helix (HLH) and leucine zipper (LZ) domains flanked by an upstream basic region. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. TFE3 is ubiquitously expressed and can directly associates with DNA as either homodimers or heterodimers formed with two related proteins, TFEB or TFEC. TFE3 binds to and activates the microE3 motif of the immunoglobulin heavy-chain enhancer to induce B-cell-specific gene transcription and DNA recombination. TFEB is expressed at low levels in the embryo but at high levels in the trophoblast cells of the placenta, where it plays a critical role in regulating normal vascularization of the placenta. Recently, Smith et al. have proven that the exit from pluripotency is gated by intracellular redistribution of the bHLH transcription factor TFE3 (Cell 153, 335–347, April 11, 2013).

免疫原

linear peptide corresponding to Human TFE3.

アプリケーション

Research Category
幹細胞研究

Research Sub Category
多能性及び初期分化

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory on TFE3 overexpressing cell lines, epiblast and primitive endodermal cells (Smith, A., et al. (2013). Cell. 153, 335–347).

This Anti-TFE3 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of TFE3.

品質

Evaluated by Western Blot in a HEK293T cell lysate.
Western Blot Analysis: 2 µg/mL of this antibody detected TFE3 in 10 µg of HEK293T cell lysate.
Uncharacterized band(s) may appear in some lysates with longer exposure times.

ターゲットの説明

~ 65 kDa observed

物理的形状

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS, 50% glycerol, and 0.05% sodium azide

保管および安定性

Store at -20°C

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2