ABE1338

Anti-METTL14 Antibody

from rabbit, purified by affinity chromatography

• 別名: N6-adenosine-methyltransferase subunit METTL14, m6A methyltransferase METTL14, Methyltransferase-like protein 14
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: mouse, human
• 化学種の反応性（ホモロジーによる予測）: zebrafish (based on 100% sequence homology), rat (based on 100% sequence homology)
• テクニック: western blot: suitable
• NCBIアクセッション番号: NP_066012
• UniProtアクセッション番号: Q9HCE5
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... METTL14(57721)

詳細

詳細

N6-adenosine-methyltransferase subunit METTL14 (EC 2.1.1.62; UniProt Q9HCE5; also known as m6A methyltransferase METTL14, Methyltransferase-like protein 14) is encoded by the METTL14 (also known as KIAA1627) gene (Gene ID 57721) in human. N(6)-Methyladenosine (m(6)A) represents the most prevalent internal modification in messenger and long noncoding RNAs. This reversible modification is installed by the m(6)A methylation "writers" and remomved by demethylases that serve as "erasers. In addition, methyl-specific binding proteins, primarily of the YTH-domain family, function as "readers" and mediate the effect by binding to modified transcripts. METTL14 is a component of the nuclear methyltransferase complex (writers) composed of METTL3, METTL14, and WTAP. At least 2 demethylases (erasers), ALKBH5 and FTO, that can catalyze the reverse reaction. The roles of m(6)A in cell fates regulation and embryonic development highlight the existence of another layer of epigenetic regulation at the RNA level.

特異性

The immunogen sequence is present in human METTL14 (UniProt Q9HCE5) and both of murine METTL14 spliced isoforms (Uniprot Q3UIK4).

免疫原

KLH-conjugated linear peptide corresponding to an internal sequence from the C-terminal half of human METTL14.

アプリケーション

This Anti-METTL14 Antibody is validated for use in Western Blotting for the detection of METTL14.

品質

Evaluated by Western Blotting in Raji nuclear extract.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected METTL14 in 10 µg of Raji nuclear extract.

ターゲットの説明

~56 kDa observed. 52.15 kDa (human), 52.12/40.72 kDa (mouse isoform 1/2) calculated. Uncharacterized band(s) may appear in some lysates.

その他情報

Concentration: Please refer to lot specific datasheet.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable