ABS1531

Anti-AGS3/GPSM1 Antibody

serum, from rabbit

• 別名: G-protein-signaling modulator 1, Activator of G-protein signaling 3
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: serum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 化学種の反応性: mouse, rat
• テクニック: immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBIアクセッション番号: NP_653346
• UniProtアクセッション番号: Q9R080
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: rat ... Gpsm1(246254)

詳細

詳細

G-protein-signaling modulator 1 (UniProt Q9R080; also known as Activator of G-protein signaling 3) is encoded by the Gpsm1 (also known as Ags3) gene (Gene ID 246254) in rat species. AGS3/GPSM1 is an evolutionarily conserved protein with four G-protein regulatory (GPR) GoLoco motifs (a.a. 493-515, 546-568, 594-616, 628-650 of murine GPSM1) and functions as a guanine nucleotide dissociation inhibitor (GDI). GPSM1 increases heteromeric polycystin-1/polycystin-2 ion channel activity via Gβγ subunits. GPSM1 is involved in regulating mitotic spindle orientation, cell polarity, and adenylyl cyclase activity. GPSM1 plays a role in renal epithelial cell repair following renal injury and GPSM1 induction in polycystic kidney disease (PKD) and may be a critical modulator of the renal cystogenic process.

特異性

This polyclonal antiserum immunostained predominantly renal collecting ducts of postnatal P12 ADPKD mice. Immunogen peptide blocking eliminated antibody stianing (Kwon, M., et al. (2012). Proc. Natl. Acad. Sci. U. S. A. 109(52):21462-21467). This antiserum targets a STK11/LKB1-interacting region sequence present in rat AGS3/GPSM1 isoform 1 and isoform 4 (isoform Long), 28% present in isoform 3 (Short-2), but completely absent from isoforms 2 (Short-1) reported by UniProt (Q9R080). Target sequence is also present in mouse isoforms 1, 3, 4, and 5, but absent from isoform 2 ( (UniProt Q6IR34).

免疫原

Epitope: STK11/LKB1-interacting region.

Linear peptide corresponding to a sequence within the STK11/LKB1-interacting region of rat AGS3/GPSM1.

アプリケーション

Research Category
細胞シグナル伝達

Research Sub Category
GPCR、cAMP/cGMP及びカルシウムシグナル伝達

Immunofluorescence Analysis: A representative lot immunostained predominantly renal collecting ducts by fluorescent immunohistochemistry using kidney sections from postnatal P12 Pkd1v/v ADPKD mice (Kwon, M., et al. (2012). Proc. Natl. Acad. Sci. U. S. A. 109(52):21462-21467).
Immunohistochemistry Analysis: A representative lot detected a time-dependent induction of AGS3/GPSM1 immunoreactivity co-localized with that of the proliferation marker Ki-67 in formalin-fixed, paraffin-embedded kidney sections from rats following ischemia-reperfusion injury (IRI) initiated by a 30-min occlusion of renal blood flow via bilateral renal artery clamping (Regner, K.R., et al. (2011). FASEB J. 25(6):1844-1855).
Western Blotting Analysis: A representative lot detected developmental stage-dependent renal AGS3/GPSM1 expression level. High level was seen in E13.5 embryonic kidney and greatly reduced among postnatal mice with low-to-undetectable levels around postnatal day P28. Postnatal renal AGS3/GPSM1 levels were found elevated and remained detectable up to P28 among Pkd1v/v autosomal dominant polycystic kidney disease (ADPKD) mice expressing noncleavable polycystin-1/PC1 (Kwon, M., et al. (2012). Proc. Natl. Acad. Sci. U. S. A. 109(52):21462-21467).
Western Blotting Analysis: A representative lot detected much higher AGS3/GPSM1 expression level in brain than kidney from postnatal day 19 (P19) wild-type or Pkd1v/v ADPKD mice (Kwon, M., et al. (2012). Proc. Natl. Acad. Sci. U. S. A. 109(52):21462-21467).
Western Blotting Analysis: A representative lot detected a time-dependent renal AGS3/GPSM1 induction in rats following ischemia-reperfusion injury (IRI) initiated by a 40-min occlusion of renal blood flow (Regner, K.R., et al. (2011). FASEB J. 25(6):1844-1855).

This Anti-AGS3 Antibody is validated for use in Western Blotting, Immunofluorescence, Immunohistochemistry (Paraffin) for the detection of GPSM1.

品質

Evaluated by Western Blotting in embryonic mouse brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected AGS3/GPSM1 in 10 µg of E13-14 embryonic mouse brain tissue lysate.

ターゲットの説明

~74/25 kDa oberved. 74.44 kDa (rat isoform 1), 72.05 kDa (rat isoform 4; isoform Long), and 25.08 kDa (rat isoform 3; isoform Short-2), 74.36/71.93/33.56/78.11 kDa (mouse isoform 1/3/4/5) calculated. Uncharacterized band(s) may appear in some lysates.

物理的形状

Unpurified.

Rabbit polyclonal antibody serum with 0.05% sodium azide.

保管および安定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable