MAB2247-I

Anti-Integrin β1 Antibody, clone 12G10

clone 12G10, from mouse

• 別名: Integrin beta-1, CD29, Glycoprotein Iia, GPIIA, Integrin VLA-4 subunit beta, Very late activation protein, beta polypeptide
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• クローン: 12G10, monoclonal
• application: FACS; IF; WB
• 化学種の反応性: human
• テクニック: flow cytometry: suitable; immunofluorescence: suitable; western blot: suitable
• citations: 1

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 12G10, monoclonal
• 化学種の反応性: human
• テクニック: flow cytometry: suitable; immunofluorescence: suitable; western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NM_002211
• UniProtアクセッション番号: P05556
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... ITGB1(3688)

詳細

詳細

Integrin beta-1 (UniProt P05556; also known as CD29, Glycoprotein Iia, GPIIA, Integrin VLA-4 subunit beta, Very late activation protein, beta polypeptide) is encoded by the ITGB1 (also known as FNRB, MDF2, MSK12, VLA-BETA, VLAB) gene (Gene ID 3688) in human. Integrin beta-1 is a 130 kDa transmembrane glycoprotein that interact with various integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptor complexes that bind to specific extracellular matrix proteins. Integrin receptors regulate a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor growth and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to the activation of signal transduction pathways and regulation of gene expression.

免疫原

Purified human β1 Integrin preparation from HT1080 fibrosarcoma cell extract

アプリケーション

Research Category
細胞骨格

Research Sub Category
ECMタンパク質

This Anti-Integrin β1 Antibody, clone 12G10 is validated for use in Western Blotting, Flow Cytometry, Immunofluorescence for the detection of Integrin β1.

Western Blot Analysis: 0.5 µg/mL from a representative lot detected Integrin β1 in 10 µg of Integrin purified protein.

Immunofluorescence Analysis: A representative lot was used in an independent laboratory in mouse lung tissue. (Chen P., et al. (2009). PLoS ONE. 4(8):e6565.)

品質

Evaluated by Flow Cytometry in HeLa cells.

Flow Cytometry Analysis: 1 µg of this antibody detected Integrin β1 in HeLa cells.

ターゲットの説明

~192 kDa observed

関連事項

Replaces: MAB2247

物理的形状

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in PBS containing 0.1% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
HeLa cells

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable