MABE146

Anti-5-methylcytosine Antibody, clone 33D3

clone 33D3, from mouse

• 別名: methylcytosine, methylcytidine
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• クローン: 33D3, monoclonal
• application: DB; FACS; IF; IHC; Southern blotting; methylated DNA immunoprecipitation (MeDIP)
• 化学種の反応性: human, mouse
• テクニック: Southern blotting: suitable; dot blot: suitable; flow cytometry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); methylated DNA immunoprecipitation (MeDIP): suitable
• citations: 41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 33D3, monoclonal
• 化学種の反応性: human, mouse
• テクニック: Southern blotting: suitable; dot blot: suitable; flow cytometry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); methylated DNA immunoprecipitation (MeDIP): suitable
• アイソタイプ: IgG1λ
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

5-Methylcytosine is a modified base found in the DNA of higher eukaryotes. Methylation of DNA is known to be involved in the control of gene expression and is mediated by a class of enzymes known as DNA methyltransferases (DNMT’s). Appropriate cytosine methylation is essential for normal development and maintenance of mammalian cells. Alternations in DNA methylation patterns is often associated with cancers with the turmor DNA being hypomethylated compared to DNA from normal tissues.

特異性

Predicted to react with multiple species.

This antibody detects 5-Methylcytosine, but not unmethylated cytosine.

免疫原

Ovalbumin-conjugated 5-methylcytosine

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Research Sub Category
エピジェネティクス

IF
Habib, M., et al. (1999). Exp Cell Res. 249:46-53

IHC-P
Hernadez-Blazquez, F.J., et al. (2000). Gut. 47:689-693

Flow Cytometry
Reynaud, C.C., et al. (1992). Cancer Lett. 61(3):255-262

MeDIP
Keshet, I., et al. (2006). Nat Genet. 38(2):149-153

Use Anti-5-methylcytosine Antibody, clone 33D3 (mouse monoclonal antibody) validated in DB, IF, SB, IHC(P), Flow Cytometry, MeDIP to detect 5-methylcytosine also known as methylcytosine, methylcytidine.

品質

Evaluated by Dot Blot in methylated and unmethylated BRCA1.

Dot Blot Analysis: 1 µg/mL of this antibody detected methylcytosine in methylated and unmethylated BRCA1.

ターゲットの説明

Detects methylated cytosine

関連事項

Replaces: MABE527

物理的形状

Protein A

Format: Purified

Purified mouse monoclonal IgG1λ in buffer containing PBS and 0.05% sodium azide.

保管および安定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

アナリシスノート

Control
Methylated and unmethylated BRCA1

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable