MABN1580

Anti-beta-Arrestin-2 Antibody, clone 5E12.1

clone 5E12.1, from mouse

• 別名: Arrestin beta-2, beta-Arrestin-2
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• クローン: 5E12.1, monoclonal
• application: IHC; WB
• 化学種の反応性: human, rat
• テクニック: immunohistochemistry: suitable (paraffin); western blot: suitable

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 5E12.1, monoclonal
• 化学種の反応性: human, rat
• テクニック: immunohistochemistry: suitable (paraffin); western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_001244257
• UniProtアクセッション番号: P32121
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... ARRB2(409)

詳細

詳細

Beta-arrestin-2 (UniProt P32121; also known as Arrestin beta-2) is encoded by the ARRB2 (also known as ARB2, ARR2, BARR2) gene (Gene ID 409) in human. β-Arrestin 1 and 2 are cytosolic adapter proteins originally identified as negative regulators of G protein-coupled receptor (GPCR) signaling through receptor desensitization and internalization. It is now known that these multifunctional scaffold proteins also elicit G-protein-independent signalling pathways by linking the activated GPCRs to multiple signaling molecules, such as ERK1/2, Akt, and Src kinases. In addtion to GPCRs, β-arrestins are also involved in receptor tyrosine kinase (RTK) signalling pathways, and β-arrestin-2 is reported to link Src and Akt to insulin receptor (IR) to ensure a full Akt activation. Although β-arrestin-1 and β-arrestin-2 share a high degree of structural homology, they do display specific functions in several physiological processes. β-arrestins can adopt multiple conformations to display distinct sets of interacting domains. Recruitment of β-arrestins to a specific GPCR results in the formation of distinct β-arrestin scaffolds, allowing a high level of specificity for a given agonist.

特異性

Expected to react with spliced isoforms 1, 4, and 5 reported by UniProt (P32121). Reactivity toward spliced isoforms 2 & 3 has not been determined.

免疫原

Epitope: C-terminal region.

KLH-conjugated linear peptide corresponding to a sequence in the C-terminal region of human beta-Arrestin-2.

アプリケーション

Research Category
ニューロサイエンス

Research Sub Category
神経シグナリング

Anti-beta-Arrestin-2 Antibody, clone 5E12.1 is an antibody against beta-Arrestin-2 for use in Western Blotting, Immunohistochemistry (Paraffin).

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected beta-Arrestin-2 in rat testis and human cerebral cortex & liver tissues.

品質

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected beta-Arrestin-2 in 200 µg of HeLa cell lysate.

ターゲットの説明

~52 kDa observed. 46.11 kDa (isoform 1), 47.27 kDa (isoform 2), 45.56 kDa (isoform 3), 48.02 kDa (isoform 4), and 4439 kDa (isoform 5) calculated.

物理的形状

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable