MABN1602

Anti-PIT-1 (POU1F1) Antibody, clone 8B6.1

clone 8B6.1, from mouse

• 別名: Pituitary-specific positive transcription factor 1, GHF-1, Growth hormone factor 1, PIT-1
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• クローン: 8B6.1, monoclonal
• application: WB
• 化学種の反応性: human
• テクニック: western blot: suitable

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 8B6.1, monoclonal
• 化学種の反応性: human
• テクニック: western blot: suitable
• アイソタイプ: IgG2aκ
• NCBIアクセッション番号: NP_000297
• UniProtアクセッション番号: P28069
• 輸送温度: ambient
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... POU1F1 (5449)

詳細

詳細

Pituitary-specific positive transcription factor 1 (UniProt P28069; also known as GHF-1, Growth hormone factor 1, PIT-1) is encoded by the POU1F1 (also known as CPHD1, GHF1, PIT1) gene (Gene ID 5449) in human. PIT-1 is a transcription factor that serves as a master regulator of lineage commitment and expansion during the anterior pituitary development process that drives regional expansion and functional differentiation of five distinct hormone-producing cell lineages, including somatotropes, lactotropes, thyrotropes, corticotropes, and gonadotropes. Loss of Pit-1 expression results in a combined pituitary hormone deficiency (CPHD) syndrome reflecting the absence of lactotropes, somatotropes, and a subset of thyrotropes. Although originally characterized as pituitary-specific, PIT-1 is also expressed in human breast. PIT-1 upregulation in breast tumors increases cell proliferation and the expression of two breast cancer-related hormones, growth hormone (GH) and prolactin (PRL), leading to MMPs upregulation and breast cancer metastasis. Human POU1F1 gene encodes two alternatively spliced isoforms, where Pit-1A (GHF-2) a.a. 47-73 is truncated to a single Thr47 residue in Pit-1B (GHF-1). Both PIT-1 isoforms contain a PIT-1 POU-specific domain (a.a. 150-224 of Pit-1A or a.a. 124-198 of Pit-1B) and a DNA-binding Homeobox (a.a. 240-299 of Pit-1A or a.a. 214-273 of Pit-1B).

特異性

Clone 8B6.1 epitope lies within an internal region present in both human PIT-1 spliced isoform A (Pit-1A, GHF-2) and isoform B (Pit-1B, GHF-1).

免疫原

GST-tagged recombinant human PIT-1 internal fragment.

アプリケーション

Anti-PIT-1 (POU1F1), clone 8B6.1 Antibody, Cat. No. MABN1602, is a highly specific mouse monoclonal antibody, that targets POU1F1 and has been tested in Western Blotting.

品質

Evaluated by Western Blotting in human pituitary tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PIT-1/POU1F1 in 10 µg of human pituitary tissue lysate.

ターゲットの説明

~33 kDa observed. 35.67 kDa (isoform A, Pit-1A, GHF-2) and 32.91 kDa (isoform B, Pit-1B, GHF-1) calculated. Uncharacterized bands may be observed in some lysate(s).

物理的形状

Format: Purified

その他情報

Concentration: Please refer to lot specific datasheet.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable